AB206870

Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] - Synaptic Marker

Name: Anti-Synaptophysin Alexa Fluor® 555 antibody [YE269] (ab206870) Rabbit monoclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB206870
Price: 660 USD
Availability: InStock
Rating: 3 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(4 Publications)

Rabbit Recombinant Monoclonal Synaptophysin antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 4 publications.

View Alternative Names

Synaptophysin, Major synaptic vesicle protein p38, SYP

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB206870)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB206870)

ab206870 staining Synaptophysin in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206870 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Carrier free

Anti-Synaptophysin antibody [YE269] - BSA and Azide free
Unconjugated

Anti-Synaptophysin antibody [YE269] - Synaptic Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] - Synaptic Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Synaptophysin antibody [YE269] - Synaptic Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] - Synaptic Marker
HRP

HRP Anti-Synaptophysin antibody [YE269] - Synaptic Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

YE269

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Donkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

See full target information SYP

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:622001 PubMed33737929

2021

Repurposing of the Antiepileptic Drug Levetiracetam to Restrain Neuroendocrine Prostate Cancer and Inhibit Mast Cell Support to Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Sulsenti,Barbara Frossi,Lucia Bongiovanni,Valeria Cancila,Paola Ostano,Irene Fischetti,Claudia Enriquez,Francesca Guana,Giovanna Chiorino,Claudio Tripodo,Carlo E Pucillo,Mario P Colombo,Elena Jachetti

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 18:309-325 PubMed33118131

2020

A Nitroalkene Benzoic Acid Derivative Targets Reactive Microglia and Prolongs Survival in an Inherited Model of ALS via NF-κB Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Sofía Ibarburu,Mariángeles Kovacs,Valentina Varela,Jorge Rodríguez-Duarte,Mariana Ingold,Paulina Invernizzi,Williams Porcal,Ana Paula Arévalo,Karen Perelmuter,Mariela Bollati-Fogolín,Carlos Escande,Gloria V López,Peter King,Ying Si,Yuri Kwon,Carlos Batthyány,Luis Barbeito,Emiliano Trias

ACS biomaterials science & engineering 6:5811-5822 PubMed33320550

2020

Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks.

Applications

Unspecified application

Species

Unspecified reactive species

Brian J O'Grady,Kylie M Balotin,Allison M Bosworth,P Mason McClatchey,Robert M Weinstein,Mukesh Gupta,Kara S Poole,Leon M Bellan,Ethan S Lippmann

JCI insight 2: PubMed29046475

2017

Evidence for mast cells contributing to neuromuscular pathology in an inherited model of ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Emiliano Trias,Sofía Ibarburu,Romina Barreto-Núñez,Valentina Varela,Ivan C Moura,Patrice Dubreuil,Olivier Hermine,Joseph S Beckman,Luis Barbeito

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] - Synaptic Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB206870)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise