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AB312912

Alexa Fluor® 555 Anti-T-bet / Tbx21 antibody [EPR9302]

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Rabbit Recombinant Monoclonal T-bet / Tbx21 antibody - conjugated to Alexa Fluor® 555. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

TBET, TBLYM, TBX21, T-box transcription factor TBX21, T-box protein 21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYM

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-T-bet / Tbx21 antibody [EPR9302] (AB312912)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-T-bet / Tbx21 antibody [EPR9302] (AB312912)

Immunohistochemical analysis of paraffin-embedded Hodgkin's lymphoma tissue labelling T-bet / Tbx21 with ab312912 at 1/50 (10.0 µg/ml). Nucleus staining on human hodgkin's lymphoma. The section was incubated with ab312912 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-T-bet / Tbx21 antibody [EPR9302] (AB312912)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-T-bet / Tbx21 antibody [EPR9302] (AB312912)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling T-bet / Tbx21 with ab312912 at 1/50 (10.0 µg/ml). Negative control : no staining on human skeletal muscle. The section was incubated with ab312912 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9302

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

T-bet also known as Tbx21 is a T-box transcription factor that plays an important role in regulating immune responses. T-bet has a molecular mass of approximately 58 kDa. It is extensively expressed in T-helper 1 (Th1) cells and natural killer (NK) cells facilitating these cells' development and function. T-bet guides the transcriptional activities necessary for cellular differentiation and immune response by binding to specific DNA sequences. Its expression is tightly controlled reflecting its central role in immune regulation. "9d bet" is also referenced in scientific research although its specific context requires precise understanding within a given study.
Biological function summary

This transcription factor is pivotal for manufacturing interferon-gamma (IFN-γ) initiating and sustaining Th1 cell immunity. T-bet doesn't act alone; it functions as part of larger protein complexes interacting with other transcription factors and cofactors to exert its effects. The capacity to influence IFN-γ production makes T-bet a significant player in immune responses helping coordinate responses against intracellular pathogens. Moreover the expression of T-bet is not limited solely to T cells but also impacts other immune cells like CD8+ T cells and B cells. Anti-bet or anti-T antibodies frequently target T-bet in research to explore immune system intricacies better.

Pathways

T-bet integrates into the immune signaling network through pathways such as the JAK-STAT pathway and the Th1 differentiation pathway. It activates transcription of the IFNG gene working closely with related proteins like STAT4. T-bet's influence extends as it cooperates with STAT1 enabling a feed-forward loop that amplifies and stabilizes Th1 responses. "Bet t products" could refer to those derived from such transcriptional activation. Additionally T-bet involvement goes beyond transcriptional regulation affecting cytokine transport and secretion processes vital for an effective immune response.

Researchers have linked T-bet to autoimmune diseases and infectious diseases. Aberrant expression of T-bet can lead to enhanced Th1 responses intensifying autoimmune conditions such as multiple sclerosis (MS). Its role in diseases connects it with NF-κB pathways and other transcription factors influencing inflammatory processes. Furthermore "anti bet" studies often involve understanding T-bet's role in infectious diseases seeking to manipulate its function to adjust immune responses in chronic infections. Studies targeting its pathways or interactions with disease-specific proteins aim to develop therapeutic strategies turning an element of dysregulation into a novel treatment approach.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed : 10761931). Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG (PubMed : 33296702). Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed : 27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses; controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (By similarity). Required for the correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells (PubMed : 33296702).
See full target information TBX21
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Product promise

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For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
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Anti-T-bet / Tbx21 antibody [EPR9302] - BSA and Azide free

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Alternative Version
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