Rabbit Recombinant Monoclonal TMEM16A antibody - conjugated to Alexa Fluor® 555.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Calcium-activated chloride channel (CaCC) (PubMed:20056604, PubMed:22178883, PubMed:22946059, PubMed:32487539). Plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression (PubMed:28963502). Required for basal and ATP-dependent mucus secretion in airways and intestine, probably by controlling exocytosis of mucus-filled granules by providing Ca(2+) to an apical signaling compartment (By similarity). Contributes to airway mucus expression induced by interleukins IL3 and IL8 and by the asthma-associated protein CLCA1 and is required for expression of mucin MUC5AC (PubMed:33026825). However, was shown in another study not to be required for MUC5AC expression (PubMed:31732694). Plays a role in the propagation of Ca(2+) waves in Kolliker's organ in the cochlea and contributes to the refinement of auditory brainstem circuitries prior to hearing onset (By similarity). In vomeronasal sensory neurons, modulates spontaneous firing patterns in the absence of stimuli as well as the firing pattern of pheromone-evoked activity (By similarity). Responsible for calcium-activated chloride channel activity in type I taste cells of the vallate papillae (By similarity). Acts as a heat sensor in nociceptive neurons (By similarity). In dorsal root ganglion neurons, plays a role in mediating non-histaminergic Mas-related G-protein coupled receptor (MRGPR)-dependent itching, acting as a downstream effector of MRGPRs (By similarity). In the developing brain, required for the Ca(2+)-dependent process extension of radial glial cells (By similarity). Isoform 4. Calcium-activated chloride channel (CaCC). Contributes to calcium-activated chloride secretion in human sweat gland epithelial cells. Shows increased basal chloride permeability and decreased Ca(2+)-induced chloride permeability. Isoform 5. Calcium-activated chloride channel (CaCC). Shows increased sensitivity to intracellular Ca(2+).
DOG1, ORAOV2, TAOS2, TMEM16A, ANO1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2
Rabbit Recombinant Monoclonal TMEM16A antibody - conjugated to Alexa Fluor® 555.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
TMEM16A also known as ANO1 is a transmembrane protein functioning as a calcium-activated chloride channel. It plays a significant role in the regulation of membrane potential and fluid secretion. TMEM16A has a molecular mass of approximately 114 kDa. This protein is expressed in various tissues including airway epithelial cells intestinal epithelium and smooth muscle cells. Its expression is also noticeable in parts of the nervous system indicating its widespread physiological importance.
TMEM16A contributes to processes such as epithelial fluid transport and smooth muscle contraction. It is an integral component of a calcium-sensitive chloride conductance. This channel regulates ion transport across epithelial cell membranes influencing fluid secretion in airways and gastrointestinal tracts. In blood vessels TMEM16A helps control vascular tone by mediating smooth muscle cell contraction which is dependent on intracellular calcium levels.
TMEM16A interacts with key cellular signaling cascades especially the calcium signaling pathway. This interaction has critical implications for chloride conductance in epithelial cells and smooth muscle function. TMEM16A is linked with proteins like calcium/calmodulin-dependent protein kinase II (CaMKII) which further modulates its activity. Additionally TMEM16A is implicated in pathways regulating mucin secretion which involves other proteins like cystic fibrosis transmembrane conductance regulator (CFTR).
TMEM16A plays a role in respiratory diseases like asthma and cystic fibrosis where dysregulation in chloride channels leads to abnormal mucus secretion and airway obstruction. It also relates to hypertension due to its role in vascular tone modulation. Research shows TMEM16A interacts with proteins like CFTR in cystic fibrosis affecting chloride transport and fluid balance. In hypertension its interaction with calcium-sensing proteins further complicates vascular responsiveness and pressure regulation.
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