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AB312100

Alexa Fluor® 555 Anti-TPPP antibody [EPR3316]

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Rabbit Recombinant Monoclonal TPPP antibody - conjugated to Alexa Fluor® 555.

View Alternative Names

TPPP1, TPPP, Tubulin polymerization-promoting protein, 25 kDa brain-specific protein, TPPP/p25, p24, p25-alpha

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3316

Isotype

IgG

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPPP also known as Tubulin Polymerization Promoting Protein or p25 is a small protein with an approximate mass of 25 kDa. TPPP is expressed mainly in the brain particularly abundant in oligodendrocytes. This protein plays an important role in regulating the polymerization of tubulin into microtubules. Through its binding to tubulin TPPP stabilizes microtubule structures facilitating proper microtubule assembly and maintenance. The modulation of these structures is essential for cellular operations like intracellular transport and cell division.
Biological function summary

The protein participates in the dynamics of cytoskeletal elements critical for maintaining cell shape and function. TPPP is part of cytoskeletal networks where it contributes to cellular integrity in neurons. TPPP's activity influences the formation and maintenance of myelin the insulating layer enveloping nerve fibers which is important for fast and efficient nerve impulse conduction. In its role TPPP interacts cooperatively with other cytoskeletal proteins enhancing microtubule resilience and cell structural stability.

Pathways

TPPP interacts in processes involving microtubule organization and stabilization. It is integral in the MAPK/ERK signaling pathway which is prominent in regulating cellular processes like proliferation and differentiation. Additionally TPPP may interplay with proteins such as MAP2 and Tau both involved in the MAP-kinase pathways regulating microtubule dynamics indicating a collaborative network in cellular signaling and structural reinforcement in neural cells.

TPPP has correlations with neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. Abnormal regulation or aggregation of TPPP is linked to the formation of pathological protein inclusions found in these conditions. The interaction between TPPP and Alpha-synuclein is particularly notable as TPPP may regulate or enhance the aggregation of Alpha-synuclein contributing to the development of Lewy bodies in Parkinson's. Understanding these interactions and pathways is essential for developing therapeutic strategies targeting these neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed : 31522887). Acts as a microtubule nucleation factor in oligodendrocytes : specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (PubMed : 31522887, PubMed : 33831707). Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (PubMed : 31522887). Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (PubMed : 21316364, PubMed : 21995432). In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (PubMed : 17105200, PubMed : 17693641, PubMed : 18028908, PubMed : 26289831). Regulates microtubule dynamics by promoting tubulin acetylation : acts by inhibiting the tubulin deacetylase activity of HDAC6 (PubMed : 20308065, PubMed : 23093407). Also regulates cell migration : phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility (PubMed : 23093407). Plays a role in cell proliferation by regulating the G1/S-phase transition (PubMed : 23355470). Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (PubMed : 22328514).
See full target information TPPP
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