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AB319190

Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56]

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Rabbit Recombinant Monoclonal ADAM22 antibody - conjugated to Alexa Fluor® 568. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

MDC2, ADAM22, Disintegrin and metalloproteinase domain-containing protein 22, ADAM 22, Metalloproteinase-disintegrin ADAM22-3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56] (AB319190)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56] (AB319190)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling ADAM22 with ab319190 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human lung.
The section was incubated with ab319190 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56] (AB319190)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56] (AB319190)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ADAM22 with ab319190 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human cerebrum.
The section was incubated with ab319190 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56] (AB319190)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56] (AB319190)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ADAM22 with ab319190 at 1/50 (10.0 ug/ml) dilution.

Positive staining on rat cerebrum.
The section was incubated with ab319190 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56] (AB319190)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ADAM22 antibody [EPR28037-56] (AB319190)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ADAM22 with ab319190 at 1/50 (10.0 ug/ml) dilution.

Positive staining on mouse cerebrum.
The section was incubated with ab319190 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28037-56

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAM22 also known as ADAM 22 or ADAM 2 is a protein that functions mechanically as a transmembrane receptor involved in cell-cell and cell-matrix interactions. This protein does not exhibit protease activity unlike other members of the ADAM family which distinguishes its functional role. ADAM22 has a mass of approximately 100 kDa. It is expressed predominantly in the central nervous system particularly in brain tissues where it plays a significant role in neuronal signaling.
Biological function summary

ADAM22 affects the nervous system's development and function by mediating synaptic formation and maintenance. It belongs to a protein complex with LGI1 which also interacts with other proteins on the synaptic membrane facilitating communication between neurons. The LGI1-ADAM22 complex reportedly regulates ion channels and other synaptic proteins impacting neurotransmission and synaptic plasticity essential for cognitive processes.

Pathways

ADAM22 acts within the neuropharmacological and synaptogenesis pathways. It interacts closely with LGI1 and PSD95 pivotal proteins in developing synaptic signaling platforms. These pathways influence neurotransmitter release and receptor clustering fundamental for effective neural circuit function. The disruption of this pathway or its elements can lead to neurological impairments and highlight ADAM22's role in maintaining neural integrity.

ADAM22 connects to neurological conditions such as epilepsy and glioma. Mutations or deficiencies in the LGI1-ADAM22 interaction have been linked to epilepsy affecting ion channel regulatory actions. In glioma altered expression of ADAM22 may play a role in tumor progression influenced by interactions with integrins and growth factors. Therefore ADAM22's presence and regulatory actions contribute to neurological and oncological disease models.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein (PubMed : 19692335). Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.
See full target information ADAM22
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Complementary Products

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Sandwich ELISA - Human ADAM22 ELISA Kit- Extracellular (AB324133)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com