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Rabbit Recombinant Monoclonal Alpha-synuclein phospho S129 antibody - conjugated to Alexa Fluor® 568. Suitable for IHC-P, IHC-Fr and reacts with Human samples.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB307166), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB307166), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB307166), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB307166), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 568
Excitation/Emission
Ex: 578nm, Em: 603nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-Fr
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

Recommended products

Rabbit Recombinant Monoclonal Alpha-synuclein phospho S129 antibody - conjugated to Alexa Fluor® 568. Suitable for IHC-P, IHC-Fr and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 568
Excitation/Emission
Ex: 578nm, Em: 603nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1536Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab307166), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab307166)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Human Parkinson's substantia nigra (fresh) tissue labeling Alpha-synuclein (phospho S129) with ab307166 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining on human Parkinson's substantia nigra. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307166 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre. The nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab307166), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab307166)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling Alpha-synuclein (phospho S129) with ab307166 at 1/50 (10.0 ug/ml) followed by a at dilution. Negative control: no staining on human skeletal muscle.The section was incubated with ab307166 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab307166), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab307166)

    Immunohistochemical analysis of paraffin-embedded Human Parkinson's di tissue labeling Alpha-synuclein (phospho S129) with ab307166 at 1/50 (10.0 ug/ml) followed by a at dilution. Positive staining on human Parkinson's disease.The section was incubated with ab307166 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab307166), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab307166)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Human skeletal muscle (fresh) tissue labeling Alpha-synuclein (phospho S129) with ab307166 at 1/50 (10.0 ug/ml) dilution (Green). Negative control: confocal image showing no staining on human skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307166 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre. The nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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