JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325786

Alexa Fluor® 568 Anti-ATP6V1B2 antibody [EPR28916-13]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ATP6V1B2 antibody - conjugated to Alexa Fluor® 568. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

ATP6B2, VPP3, ATP6V1B2, V-ATPase subunit B 2, Endomembrane proton pump 58 kDa subunit, HO57, Vacuolar proton pump subunit B 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ATP6V1B2 antibody [EPR28916-13] (AB325786)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ATP6V1B2 antibody [EPR28916-13] (AB325786)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP6V1B2 with ab325786 at 1/50 (10.0 µg/ml) dilution

Positive staining on human kidney.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ATP6V1B2 antibody [EPR28916-13] (AB325786)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-ATP6V1B2 antibody [EPR28916-13] (AB325786)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ATP6V1B2 with ab325786 at 1/50 (10.0 µg/ml) dilution

Low expression tissue : almost no staining on human cardiac muscle.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Unconjugated

    Anti-ATP6V1B2 antibody [EPR28916-13]

  • Carrier free

    Anti-ATP6V1B2 antibody [EPR28916-13] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28916-13

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for mouse TMA.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1B2 also known as V-type proton ATPase subunit B2 is an essential component of the vacuolar ATPase (V-ATPase) complex. It weighs approximately 56 kDa. This protein is responsible for the hydrolysis of ATP supplying energy for proton translocation across membranes. ATP6V1B2 is heavily expressed in various tissues including the brain and kidney. As part of the V1 domain of the V-ATPase it plays an important role in regulating intracellular pH.
Biological function summary

ATP6V1B2 contributes to several key processes by enabling proton transport critical for organelle acidification. It is a component of the V-ATPase complex a multi-subunit complex important for the acidification of intracellular compartments. Organelle acidification affects processes like protein degradation neurotransmitter loading and receptor-mediated endocytosis. ATP6V1B2 specifically aids in the stabilization and assembly of the V-ATPase complex ensuring effective proton pumping activity in diverse cellular environments.

Pathways

ATP6V1B2 plays a significant role in both endocytic and autophagic pathways. The endocytic pathway integrates proteins such as clathrin and dynamin which are important for the internalization and recycling of membrane components. In autophagy ATP6V1B2 assists in the fusion of autophagosomes with lysosomes influencing cellular homeostasis and response to nutrient deprivation. These pathways are integral for maintaining cellular energy balance growth and survival.

ATP6V1B2 has associations with conditions such as dominant and recessive cutis laxa type II and Zimmermann-Laband syndrome. These diseases often display craniofacial abnormalities and variable connective tissue symptoms. Connections with proteins like ATP6V1A have been identified which also participate in similar disorders due to shared structural roles in maintaining proper V-ATPase complex function. Understanding these links can further research into targeted therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed : 32001091). In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load (By similarity).
See full target information ATP6V1B2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com