AB210980

Alexa Fluor® 568 Anti-Cyclin A2 antibody [Y193]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cyclin A2 antibody - conjugated to Alexa Fluor® 568. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CCN1, CCNA, CCNA2, Cyclin-A2, Cyclin-A, Cyclin A

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Cyclin A2 antibody [Y193] (AB210980)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Cyclin A2 antibody [Y193] (AB210980)

ab210980 staining Cyclin A2 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210980 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cyclin A2 antibody [Y193]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cyclin A2 antibody [Y193]
Unconjugated

Anti-Cyclin A2 antibody [Y193]
Carrier free

Anti-Cyclin A2 antibody [Y193] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y193

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.

See full target information CCNA2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 39:566-579.e7 PubMed33848478

2021

Therapeutic targeting of ATR yields durable regressions in small cell lung cancers with high replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Anish Thomas,Nobuyuki Takahashi,Vinodh N Rajapakse,Xiaohu Zhang,Yilun Sun,Michele Ceribelli,Kelli M Wilson,Yang Zhang,Erin Beck,Linda Sciuto,Samantha Nichols,Brian Elenbaas,Janusz Puc,Heike Dahmen,Astrid Zimmermann,Jillian Varonin,Christopher W Schultz,Sehyun Kim,Hirity Shimellis,Parth Desai,Carleen Klumpp-Thomas,Lu Chen,Jameson Travers,Crystal McKnight,Sam Michael,Zina Itkin,Sunmin Lee,Akira Yuno,Min-Jung Lee,Christophe E Redon,Jessica D Kindrick,Cody J Peer,Jun S Wei,Mirit I Aladjem,William Douglas Figg,Seth M Steinberg,Jane B Trepel,Frank T Zenke,Yves Pommier,Javed Khan,Craig J Thomas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 568 Anti-Cyclin A2 antibody [Y193]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Cyclin A2 antibody [Y193] (AB210980)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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Product promise