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AB313001

Alexa Fluor® 568 Anti-ENT1 antibody [SP120]

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Rabbit Recombinant Monoclonal ENT1 antibody - conjugated to Alexa Fluor® 568.

View Alternative Names

ENT1, SLC29A1, Equilibrative nucleoside transporter 1, hENT1, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Solute carrier family 29 member 1, Equilibrative NBMPR-sensitive nucleoside transporter, es nucleoside transporter

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP120

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENT1 also known as Equilibrative Nucleoside Transporter 1 or SLC29A1 is a membrane protein responsible for transporting nucleosides across cell membranes. This protein weighs approximately 55 kDa and is expressed in various tissues including the brain heart and liver. The transport activity of ENT1 plays a fundamental role in maintaining cellular nucleoside levels. The efficient transport mechanisms of ENT1 highlight its significance in regulating the availability of substrates for nucleic acid synthesis.
Biological function summary

This transporter facilitates the uptake and recycling of nucleosides which are essential building blocks for DNA and RNA synthesis. ENT1 mediates the transport of adenosine an important nucleoside involved in multiple cellular functions. It operates as a component of a broader transport system that includes similar proteins like ENT2. This action contributes to cellular processes by controlling nucleoside concentrations impacting cell proliferation and metabolic activities.

Pathways

ENT1 plays a role in the purine salvage pathway and adenosine signaling. It regulates extracellular adenosine levels influencing adenosine receptor-mediated pathways that impact many physiological functions. ENT1 coordinates with proteins such as adenosine kinases to manage the intracellular balance of adenosine therefore integrating into both metabolic and signaling routes. Its role in these pathways affects energy metabolism and supports cellular responses to stress.

ENT1 has been associated with conditions such as cardiovascular diseases and neurological disorders. Altered ENT1 activity can affect adenosine levels leading to variations in blood flow and neurotransmission. In cardiovascular diseases ENT1 modulates adenosine's cardioprotective effects. In neurological contexts dysfunction of ENT1 relates to disorders involving adenosine receptor signaling where its interaction with proteins like ADA (adenosine deaminase) can influence disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed : 10722669, PubMed : 10755314, PubMed : 12527552, PubMed : 14759222, PubMed : 15037197, PubMed : 17379602, PubMed : 21795683, PubMed : 26406980, PubMed : 27995448, PubMed : 35790189, PubMed : 8986748). Functions as a Na(+)-independent transporter (PubMed : 8986748). Involved in the transport of nucleosides such as adenosine, guanosine, inosine, uridine, thymidine and cytidine (PubMed : 10722669, PubMed : 10755314, PubMed : 12527552, PubMed : 14759222, PubMed : 15037197, PubMed : 17379602, PubMed : 26406980, PubMed : 8986748). Also transports purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed : 21795683, PubMed : 27995448). Mediates basolateral nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis barrier (By similarity). Regulates inosine levels in brown adipocytes tissues (BAT) and extracellular inosine levels, which controls BAT-dependent energy expenditure (PubMed : 35790189).
See full target information SLC29A1
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