AB202540

Alexa Fluor® 568 Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)]-Nucleolar Marker

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Fibrillarin+Fibrillarin-like protein 1 antibody - conjugated to Alexa Fluor® 568. Nucleolus marker. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FIB1, FLRN, FBL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin, RNA 2'-O-methyltransferase FBLL1, Fibrillarin like 1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)]-Nucleolar Marker (AB202540)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)]-Nucleolar Marker (AB202540)

ab202540 staining Fibrillarin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202540 at 1/200 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

HRP

HRP Anti-Fibrillarin antibody+Fibrillarin-like protein 1 [EPR10823(B)] - Nucleolar Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)]-Nucleolar Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)]-Nucleolar Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)]-Nucleolar Marker
Unconjugated

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker
Carrier free

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10823(B)

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed : 24352239, PubMed : 30540930, PubMed : 32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed : 30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed : 32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed : 32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed : 24352239, PubMed : 30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information FBL

Additional targets

FBLL1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:557-566 PubMed40604277

2025

Mapping and engineering RNA-driven architecture of the multiphase nucleolus.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia A Quinodoz,Lifei Jiang,Aya A Abu-Alfa,Troy J Comi,Hongbo Zhao,Qiwei Yu,Lennard W Wiesner,Jordy F Botello,Anita Donlic,Elizabeth Soehalim,Prashant Bhat,Christiane Zorbas,Ludivine Wacheul,Andrej Košmrlj,Denis L J Lafontaine,Sebastian Klinge,Clifford P Brangwynne

PLoS pathogens 18:e1010434 PubMed35390107

2022

Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ronni A G da Silva,Wei Hong Tay,Foo Kiong Ho,Frederick Reinhart Tanoto,Kelvin K L Chong,Pei Yi Choo,Alexander Ludwig,Kimberly A Kline

Blood cancer discovery 3:194-207 PubMed35176137

2022

Integrated Genomic Analysis Identifies UBTF Tandem Duplications as a Recurrent Lesion in Pediatric Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Umeda,Jing Ma,Benjamin J Huang,Kohei Hagiwara,Tamara Westover,Sherif Abdelhamed,Juan M Barajas,Melvin E Thomas,Michael P Walsh,Guangchun Song,Liqing Tian,Yanling Liu,Xiaolong Chen,Pandurang Kolekar,Quang Tran,Scott G Foy,Jamie L Maciaszek,Andrew B Kleist,Amanda R Leonti,Bengsheng Ju,John Easton,Huiyun Wu,Virginia Valentine,Marcus B Valentine,Yen-Chun Liu,Rhonda E Ries,Jenny L Smith,Evan Parganas,Ilaria Iacobucci,Ryan Hiltenbrand,Jonathan Miller,Jason R Myers,Evadnie Rampersaud,Delaram Rahbarinia,Michael Rusch,Gang Wu,Hiroto Inaba,Yi-Cheng Wang,Todd A Alonzo,James R Downing,Charles G Mullighan,Stanley Pounds,M Madan Babu,Jinghui Zhang,Jeffrey E Rubnitz,Soheil Meshinchi,Xiaotu Ma,Jeffery M Klco

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 568 Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)]-Nucleolar Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)]-Nucleolar Marker (AB202540)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (3)

Product promise