AB201736

Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 568. Suitable for IHC-Fr, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 3 publications.

View Alternative Names

Glial fibrillary acidic protein, GFAP

1 Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201736)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201736)

IHC image of GFAP staining in a section of frozen normal rat dentate gyrus.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab201736 at 1/100 (pseudocolored in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
578 PE

PE Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
660 APC

APC Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
HRP

HRP Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
Biotin

Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
Unconjugated

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
Carrier free

Anti-GFAP antibody [EPR1034Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1034Y

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

Reacts with

Rat, Human, Mouse, Rat

Applications

IHC-Fr, Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "This product gave a positive signal in rat frozen dentate gyrus tissue fixed with 10% formaldehyde (10 min).", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:485-492 PubMed39512105

2024

Impact of 6-OHDA injection and microtrauma in the rat on local brain amyloid beta protein concentrations in the affected area.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua A Roldán-Kalil,Sara E Vendrell-Gonzalez,Natalia Espinosa-Ponce,Jadier Colón-Vasques,Jescelica Ortiz-Rivera,Vassiliy Tsytsarev,Janaina M Alves,Mikhail Inyushin

Heliyon 6:e03702 PubMed32322711

2020

Glymphatic clearance of simulated silicon dispersion in mouse brain analyzed by laser induced breakdown spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Shehzad Khan,Rachit Kumar,Sinai H C Manno,Irfan Ahmed,Alan Wing Lun Law,Raul R Cruces,Victor Ma,William C Cho,Shuk Han Cheng,Condon Lau

Brain research 1721:146306 PubMed31247207

2019

Early detection of Alzheimer's disease by peptides from phage display screening.

Applications

Unspecified application

Species

Unspecified reactive species

Jinmei Chen,Yanruo Huang,Cenjing Zhu,Qingwei Li,Yingyan Wu,Qiaofeng Liu,Qi Cheng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB201736)

Related conjugates and formulations (11)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise