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Rabbit Recombinant Monoclonal HDAC9 antibody - conjugated to Alexa Fluor® 568.

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Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Target data

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.

Alternative names

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Rabbit Recombinant Monoclonal HDAC9 antibody - conjugated to Alexa Fluor® 568.

Alternative names

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR5223

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

Supplementary info

Biological function summary

HDAC9 plays a significant role in regulating gene expression by altering the acetylation status of histones. It participates in the formation of large multiprotein complexes that target specific gene promoters. HDAC9's activity impacts various cellular processes including cell cycle progression and differentiation. It is a part of the CoREST complex which interacts with REST corepressor 1 to modulate neuronal gene expression and synaptic plasticity.

Activity summary

HDAC9 also known as Histone Deacetylase 9 is a protein involved in removing acetyl groups from histone proteins leading to chromatin condensation and transcriptional repression. HDAC9 has a molecular weight of about 127 kDa. It expresses predominantly in the brain muscle tissue and immune cells. This protein belongs to the class IIa family of histone deacetylases and has close structural similarities to other class IIa HDACs like HDAC4 HDAC5 and HDAC7.

Pathways

HDAC9 is actively involved in key cellular signaling cascades. It contributes to the Notch signaling pathway which has a profound impact on cell differentiation and fate decisions. It also plays a role in the NF-kB pathway which is critical for immune response and inflammation. Through these pathways HDAC9 interacts with proteins such as RBP-Jk in the Notch pathway and IKK complex members in the NF-kB pathway indicating its broad influence on cellular mechanisms.

Associated diseases and disorders

HDAC9 associates with several pathological conditions. It has been linked to cardiovascular diseases such as cardiac hypertrophy and heart failure due to its influence on cardiac muscle gene expression. HDAC9 also relates to various cancers including colon cancer where its dysregulation affects tumorigenesis and cell proliferation. In these contexts HDAC9 interacts with proteins like MEF2 and p300 which are pivotal in cardiac growth and cancer progression respectively.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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    Product protocols

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    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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