AB221003

Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker

Name: Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] (ab221003) | Abcam
Brand: Abcam Limited
SKU: AB221003
Price: 630 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal Iba1 antibody - conjugated to Alexa Fluor® 568. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 9 publications.

View Alternative Names

G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, iba1, iba-1, iba 1

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB221003)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB221003)

ab221003 staining Iba1 in K562 cells. The cells were fixed with 80% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab221003 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB221003)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB221003)

Overlay histogram showing K562 cells stained with ab221003 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221003, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 568 (ab209613) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

578 PE

PE Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
Unconjugated

Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
Carrier free

Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6136(2)

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in K562 cells fixed with 80% methanol (5 min)", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.

Biological function summary

Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.

Pathways

Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.

Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

See full target information AIF1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1543710 PubMed40143972

2025

The effect of Polybrominated diphenyl ethers at the fetal blood-brain-barrier: evaluation using a microphysiological system.

Applications

Unspecified application

Species

Unspecified reactive species

Sourabh Sharma,Manuel Vidal,Souvik Paul,Arum Han,Ramkumar Menon,Lauren S Richardson

Nutrients 16: PubMed39408267

2024

The Influence of Strain and Sex on High Fat Diet-Associated Alterations of Dopamine Neurochemistry in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen A Hagarty-Waite,Heather A Emmons,Steve C Fordahl,Keith M Erikson

Nature communications 15:2236 PubMed38472181

2024

Inhalation of ACE2-expressing lung exosomes provides prophylactic protection against SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Wang,Shiqi Hu,Kristen D Popowski,Shuo Liu,Dashuai Zhu,Xuan Mei,Junlang Li,Yilan Hu,Phuong-Uyen C Dinh,Xiaojie Wang,Ke Cheng

American journal of reproductive immunology (New York, N.Y. : 1989) 88:e13638 PubMed36308737

2022

Adapting an organ-on-chip device to study the effect of fetal sex and maternal race/ethnicity on preterm birth related intraamniotic inflammation leading to fetal neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren S Richardson,Nkechinyere Emezienna,Irina Burd,Brandie D Taylor,Morgan R Peltier,Arum Han,Ramkumar Menon

Nature biomedical engineering 6:791-805 PubMed35788687

2022

Exosomes decorated with a recombinant SARS-CoV-2 receptor-binding domain as an inhalable COVID-19 vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Wang,Kristen D Popowski,Dashuai Zhu,Blanca López de Juan Abad,Xianyun Wang,Mengrui Liu,Halle Lutz,Nicole De Naeyer,C Todd DeMarco,Thomas N Denny,Phuong-Uyen C Dinh,Zhenhua Li,Ke Cheng

iScience 25:104289 PubMed35573189

2022

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mio Iwasaki,Yuka Kawahara,Chikako Okubo,Tatsuya Yamakawa,Michiko Nakamura,Tsuyoshi Tabata,Yohei Nishi,Megumi Narita,Akira Ohta,Hirohide Saito,Takuya Yamamoto,Masato Nakagawa,Shinya Yamanaka,Kazutoshi Takahashi

Journal of inflammation research 14:2587-2600 PubMed34168482

2021

Genistein Attenuates Isoflurane-Induced Neuroinflammation by Inhibiting TLR4-Mediated Microglial-Polarization in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Jiang,Shoucai Xu,Yangyang Shen,Yong Xu,Yuwen Li

Nature nanotechnology 16:942-951 PubMed34140674

2021

Cell-mimicking nanodecoys neutralize SARS-CoV-2 and mitigate lung injury in a non-human primate model of COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Li,Zhenzhen Wang,Phuong-Uyen C Dinh,Dashuai Zhu,Kristen D Popowski,Halle Lutz,Shiqi Hu,Mark G Lewis,Anthony Cook,Hanne Andersen,Jack Greenhouse,Laurent Pessaint,Leonard J Lobo,Ke Cheng

Cell death and differentiation : PubMed32514048

2020

SUGT1 controls susceptibility to HIV-1 infection by stabilizing microtubule plus-ends.

Applications

Unspecified application

Species

Unspecified reactive species

Awatef Allouch,Cristina Di Primio,Audrey Paoletti,Gabrielle Lê-Bury,Frédéric Subra,Valentina Quercioli,Roberta Nardacci,Annie David,Héla Saïdi,Anna Cereseto,David M Ojcius,Guillaume Montagnac,Florence Niedergang,Gianfranco Pancino,Asier Saez-Cirion,Mauro Piacentini,Marie-Lise Gougeon,Guido Kroemer,Jean-Luc Perfettini

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB221003)

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB221003)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise