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AB312643

Alexa Fluor® 568 Anti-INPP4B antibody [EPR3108Y]

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Rabbit Recombinant Monoclonal INPP4B antibody - conjugated to Alexa Fluor® 568.

View Alternative Names

Inositol polyphosphate 4-phosphatase type II, INPP4B

  • 578 PE

    PE Anti-INPP4B antibody [EPR3108Y]

  • 660 APC

    APC Anti-INPP4B antibody [EPR3108Y]

  • HRP

    HRP Anti-INPP4B antibody [EPR3108Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-INPP4B antibody [EPR3108Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-INPP4B antibody [EPR3108Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-INPP4B antibody [EPR3108Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-INPP4B antibody [EPR3108Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-INPP4B antibody [EPR3108Y]

  • Unconjugated

    Anti-INPP4B antibody [EPR3108Y]

  • Carrier free

    Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3108Y

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INPP4B also known as inositol polyphosphate-4-phosphatase type II has a molecular mass of approximately 105 kDa. This enzyme primarily localizes in the cytoplasm but also presents in the nucleus. It catalyzes the hydrolysis of the 4-phosphate group from phosphoinositides converting phosphatidylinositol (34)-bisphosphate (PI(34)P2) to phosphatidylinositol 3-phosphate (PI3P). INPP4B expression is noticeable in various tissues including the prostate thyroid and breast suggesting its diverse roles in different physiological processes.
Biological function summary

INPP4B participates in regulating intracellular signaling pathways and maintaining cellular homeostasis. It is a negative regulator of the PI3K/AKT signaling cascade a critical pathway controlling cell growth proliferation survival and metabolism. INPP4B acts as a tumor suppressor modulating signals that impact cellular transformation and tumorigenesis. While not known to form a complex itself it indirectly influences complexes within the cascade through its regulatory functions.

Pathways

INPP4B contributes to the PI3K/AKT and mTOR pathways. It regulates PI3K/AKT signaling by decreasing levels of PI(34)P2 therefore modulating AKT activity. This action makes it an essential component in controlling mTOR signaling which affects protein synthesis and autophagy. INPP4B closely interacts with other signaling proteins like PTEN also a phosphatase which further lowers PI3K signaling by dephosphorylating PI(345)P3 to PI(45)P2.

INPP4B has notable links to cancer and insulin resistance. In some cancers like breast and prostate reduced INPP4B expression correlates with increased aggressive phenotypic markers and poor outcomes indicating its tumor-suppressive role. Its regulation of the PI3K/AKT pathway connects it to insulin signaling where dysregulated pathways can lead to insulin resistance a critical factor in type 2 diabetes. INPP4B interacts with proteins involved in these diseases like AKT and PTEN which further influence and exacerbate the pathology in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PubMed : 24070612, PubMed : 24591580). Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PubMed : 24591580). The lipid phosphatase activity is critical for tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed : 19647222, PubMed : 24070612).
See full target information INPP4B
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