Rabbit Recombinant Monoclonal JNK3 antibody - conjugated to Alexa Fluor® 568.
IgG
Rabbit
Alexa Fluor® 568
Ex: 578nm, Em: 603nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
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Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).
Mitogen-activated protein kinase 10, MAP kinase 10, MAPK 10, MAP kinase p49 3F12, Stress-activated protein kinase 1b, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, SAPK1b, JNK3A, SAPK1B, JNK3, MAPK10, PRKM10
Rabbit Recombinant Monoclonal JNK3 antibody - conjugated to Alexa Fluor® 568.
Mitogen-activated protein kinase 10, MAP kinase 10, MAPK 10, MAP kinase p49 3F12, Stress-activated protein kinase 1b, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, SAPK1b, JNK3A, SAPK1B, JNK3, MAPK10, PRKM10
IgG
Rabbit
Alexa Fluor® 568
Ex: 578nm, Em: 603nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR5693
Affinity purification Protein A
This antibody will detect will detect JNK1 (pT183), JNK2 (pT183) and JNK3 (pT221).
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
JNK1 JNK2 and JNK3 are c-Jun N-terminal kinases also known as stress-activated protein kinases (SAPKs). These kinases play significant roles in cellular stress responses. Commonly referred to by their shorter names JNK1 JNK2 and JNK3 they possess molecular weights of approximately 46 kDa to 55 kDa depending on their isoforms. These kinases are widely expressed across various tissues with JNK1 and JNK2 seen in most tissues whereas JNK3 shows more expression in neural tissues. Activation of JNKs occurs through phosphorylation at threonine 183 (T183) for JNK1 and JNK2 and threonine 221 (T221) for JNK3.
C-Jun N-terminal kinases play important roles in mediating responses to stress stimuli including cytokines and ultraviolet irradiation. They are known to be part of the MAPK signaling complex and have direct involvement in the regulation of genes connected to apoptosis and cellular proliferation. JNK1 and JNK2 in particular have broad cellular roles and influence the activity of transcription factors such as c-Jun impacting gene expression significantly. JNK3 meanwhile contributes more to neuronal apoptosis given its expression pattern.
JNK1 JNK2 and JNK3 actively engage in the MAPK signaling pathway connecting with several upstream and downstream proteins like MKK4 and MKK7 which serve as upstream kinases and c-Jun which acts downstream. The MAPK pathway is critical for translating extracellular signals into a wide range of cellular processes. The involvement of JNKs in this pathway highlights their contribution to balancing cell survival and death signals placing them alongside related proteins like ERK and p38MAPK within the signaling hierarchy.
The activation and regulation of JNK1 JNK2 and JNK3 have essential implications in neurodegenerative diseases and cancer. JNK3 in particular is associated with neurodegenerative conditions such as Alzheimer's disease due to its role in neuronal stress-induced apoptosis. Similarly aberrant JNK1 and JNK2 activation connects to various cancers through their influence on genes governing cell cycle and apoptosis often in tandem with oncogenes or tumor suppressors like p53 and Bcl-2. These associations highlight the therapeutic potential of targeting these kinases in disease treatment strategies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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