Skip to main content

Rabbit Recombinant Monoclonal LAMP1 antibody - conjugated to Alexa Fluor® 568. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Mouse samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB302802), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB302802), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB302802), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 568
Excitation/Emission
Ex: 578nm, Em: 603nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IF
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

Target data

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:15121881). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (By similarity). Also plays an important role in NK-cells cytotoxicity (PubMed:23847195). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (By similarity). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (By similarity). Also implicated in tumor cell metastasis (PubMed:2676155).

Alternative names

CD107a, Lamp-1, Lamp1, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, 120 kDa lysosomal membrane glycoprotein, CD107 antigen-like family member A, LGP-120, Lysosomal membrane glycoprotein A, P2B, LGP-A

Recommended products

Rabbit Recombinant Monoclonal LAMP1 antibody - conjugated to Alexa Fluor® 568. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 568
Excitation/Emission
Ex: 578nm, Em: 603nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21026
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

Associated diseases and disorders

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (ab302802), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (ab302802)

    LAMP1 Flow Cytometry (Intracellular) staining using rabbit Anti-LAMP1 antibody

    Flow Cytometry (Intracellular) analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells stained for LAMP1 using ab302802 at a 1/500 dilution (0.1 μg) (Red) compared to Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209613) (Black) and cells without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (ab302802), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (ab302802)

    LAMP1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-LAMP1 antibody

    Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells staining LAMP1 using ab302802 at a 1/50 dilution (10.0 μg/ml) (Red). Counterstained with Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) at a 1/200 dilution (2.5 μg/ml) (Green). Nuclear counterstain is DAPI (Blue).
    Confocal image showing cytoplasmic staining in NIH/3T3 cells.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (ab302802), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (ab302802)

    LAMP1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-LAMP1 antibody

    Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells staining LAMP1 using ab302802 at a 1/50 dilution (10.0 μg/ml) (Red). Counterstained with Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) at a 1/200 dilution (2.5 μg/ml) (Green). Nuclear counterstain is DAPI (Blue).
    Confocal image showing cytoplasmic staining in Neuro-2a cells.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com