Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691]

10 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP2 with ab303465 at 1/100 dilution (5.0 µg/mL). Positive staining is observed on human cerebrum. The section was incubated with ab303465 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins was used.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab303465 at 1/100 dilution (5.0 µg/mL). Positive staining is observed on mouse cerebrum. The section was incubated with ab303465 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins was used.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab303465 at 1/100 dilution (5.0 µg/mL). Positive staining is observed on rat cerebrum. The section was incubated with ab303465 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins was used.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labeling MAP2 with ab303465 at 1/250 dilution (2 μg/ml) (Red). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 μg/ml dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Green). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labeling MAP2 with ab303465 at 1/250 dilution (2 ug/ml) (Green). Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm, followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 μg/ml dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Green). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neurons staining MAP2 using ab303465 at a 1/250 dilution (2 μg/ml) (Red). Counterstained with Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody at a 1/500 dilution (4 μg/ml), followed by ab50113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution (2 μg/ml) (Green). Nuclear counterstain is DAPI (Blue).
  Confocal image showing positive staining in rat primary neuron.
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neurons staining MAP2 using ab303465 at a 1/250 dilution (2 μg/ml) (Red). Counterstained with Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody at a 1/500 dilution (4 μg/ml), followed by ab50113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution (2 μg/ml) (Green). Nuclear counterstain is DAPI (Blue).
  Confocal image showing positive staining in mouse primary neuron.
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue stained for MAP2 using ab303465 at a 1/100 dilution (5.0 μg/ml) (Red). Positive staining on mouse cerebrum.
  The section was incubated with Alexa Fluor® 647 Anti-CD127 antibody [EPR23747-333] ab303455 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  The immunostaining was performed on a Leica Biosystems BOND RX instrument.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue stained for MAP2 using ab303465 at a 1/100 dilution (5.0 μg/ml) (Red). Positive staining on human cerebrum.
  The section was incubated with Alexa Fluor® 647 Anti-CD127 antibody [EPR23747-333] ab303455 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  The immunostaining was performed on a Leica Biosystems BOND RX instrument.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] (ab303465)

  Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue stained for MAP2 using ab303465 at a 1/100 dilution (5.0 μg/ml) (Red). Positive staining on rat cerebrum.
  The section was incubated with Alexa Fluor® 647 Anti-CD127 antibody [EPR23747-333] ab303455 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  The immunostaining was performed on a Leica Biosystems BOND RX instrument.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).