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AB325039

Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44]

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Rabbit Recombinant Monoclonal MNDA antibody - conjugated to Alexa Fluor® 568. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Myeloid cell nuclear differentiation antigen, MNDA

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MNDA with ab325039 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human liver.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MNDA with ab325039 at 1/100 (5.0 ug/ml) dilution.

Nuclear staining on human tonsil.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling MNDA with ab325039 at 1/100 (5.0 ug/ml) dilution.

Nuclear staining on human Hodgkin's lymphoma.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling MNDA with ab325039 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control.

Cells were stained with anti-CD19 conjugated to Alexa Fluor®647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody.

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling MNDA with ab325039 at 1/50000 dilution (0.001ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : K-562 (PMID : 12112016).

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-MNDA antibody [EPR28267-44] (AB325039)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling MNDA with ab325039 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control.

Cells were stained with anti-CD56 conjugated to Brilliant Violet 421. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28267-44

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MNDA known as Myeloid Cell Nuclear Differentiation Antigen has a mass of approximately 45 kDa. Expression occurs mainly in myeloid cells including monocytes and granulocytes. It is encoded by the MNDA gene situated on chromosome 1q22. MNDA belongs to the HIN-200 family of proteins which are involved in various cellular activities. The protein contains a DNA-binding domain suggesting its role in transcriptional regulation.
Biological function summary

The function of MNDA is critical for the immune response and cellular differentiation. MNDA participates in regulating gene transcription in response to external stimuli. It operates in complex with other nuclear proteins to modulate the immune and inflammatory response aiming for a precise cellular behavior. MNDA's presence in myeloid cells signifies its role in the innate immune system contributing significantly to cell maturation and defense mechanisms.

Pathways

MNDA influences gene expression modifications tied to key immune response pathways. It has a noted involvement in the Toll-like receptor (TLR) signaling pathway where it helps mediate responses to pathogens. MNDA interacts with proteins such as TLR4 which plays a vital role in innate immunity. Furthermore MNDA participates in the interferon regulatory factor (IRF) pathway impacting type I interferon production and immune regulation.

MNDA has associations with myeloid malignancies and autoimmune conditions. Abnormal expression or mutation of MNDA might contribute to disease pathology in acute myeloid leukemia (AML) where it may function alongside proteins like RUNX1 a transcription factor important in hematopoiesis. Additionally MNDA expressions and mutations link to systemic lupus erythematosus (SLE) where its regulatory impact on the immune response may be disrupted influencing inflammatory pathways and immune system regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a transcriptional activator/repressor in the myeloid lineage. Plays a role in the granulocyte/monocyte cell-specific response to interferon. Stimulates the DNA binding of the transcriptional repressor protein YY1.
See full target information MNDA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com