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AB312482

Alexa Fluor® 568 Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

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Rabbit Recombinant Monoclonal PHF1 antibody - conjugated to Alexa Fluor® 568.

View Alternative Names

PCL1, PHF1, PHD finger protein 1, Protein PHF1, hPHF1, Polycomb-like protein 1, hPCl1

  • Unconjugated

    Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • 578 PE

    PE Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • HRP

    HRP Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • 660 APC

    APC Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

  • Carrier free

    Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14222

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to PHD finger protein 1, not paired helical filaments.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PHD finger protein 1 also known as PHF1 is a protein involved in chromatin remodeling. It weighs approximately 60 kDa. You may find PHF1 expressed in various human tissues including the brain heart and skeletal muscle which suggests its versatile roles across different cell types. This protein contains a plant homeodomain (PHD) finger motif which functions mechanically in binding to specific histone modifications notably those associated with methylated lysine residues.
Biological function summary

PHD finger protein 1 plays a role in regulating gene expression by modifying the chromatin structure. It acts as part of the PRC2 complex which is pivotal for depositing methyl marks on histone H3 at lysine 27 (H3K27me3). This modification results in transcriptional repression of target genes. Additionally PHF1 interacts with other chromatin regulators thereby contributing to the finely-tuned control of genetic programs essential for cellular differentiation and development.

Pathways

PHD finger protein 1 is involved in the Polycomb group (PcG) pathways along with the Wnt signaling pathway. PHF1 cooperates with other proteins like EZH2 which is an important enzymatic component of the PRC2 complex to mediate its repressive function on chromatin. This interaction and pathway association highlight its involvement in maintaining stem cell pluripotency and lineage specification during development.

PHD finger protein 1 associates with cancers notably endometrial cancer and sarcomas. Alterations in PHF1 expression or function influence the disease progression partly through its interaction with EZH2 leading to abnormal silencing of tumor suppressor genes. Investigations of PHF1 reveal its potential as a biomarker and therapeutic target connecting its modulation to disease outcomes and treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex : it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed : 18285464, PubMed : 23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed : 23142980). Regulates p53/TP53 stability and prolonges its turnover : may act by specifically binding to a methylated from of p53/TP53.
See full target information PHF1
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