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Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for Flow Cyt (Intra) and reacts with samples.

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Images

Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 568
Excitation/Emission
Ex: 578nm, Em: 603nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Application
Flow Cyt (Intra)
Reactivity
Reacts
Dilution info
-
Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

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Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for Flow Cyt (Intra) and reacts with samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 568
Excitation/Emission
Ex: 578nm, Em: 603nm
Form
Liquid
Clonality
Monoclonal
Clone number
EPR25A
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)

    Flow cytometric analysis of Mouse splenocytes cells labelling PSGL-1 with Alexa Fluor® 568 Anti-PSGL-1 antibody [EPR28170-27] ab323446 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Gated on viable cells.

    Cells are co-stained with CD3 conjugated to Alexa Fluor®647.

  • Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)

    Flow cytometric analysis of Mouse bone marrow cells labelling Sialoadhesin/CD169 with Alexa Fluor® 568 Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab316878 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control.

    Mouse bone marrow cells are co-stained with CD3 conjugated Alexa Fluor®647. Gated on viable cell.

  • Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)

    Flow cytometric analysis of Mouse bone marrow cells labelling Sialoadhesin/CD169 with Alexa Fluor® 568 Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab316878 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control.

    Mouse bone marrow cells are co-stained with F4/80 conjugated Alexa Fluor®647. Gated on viable cell.

  • Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)

    Flow cytometric analysis of L1CAM KO HeLa(human cervical adenocarcinoma epithelial cell, Left)

    Parental HeLa(Right) cells labelling L1CAM with Alexa Fluor® 568 Anti-L1CAM antibody [EPR23241-224] ab315361 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Gated on viable cell.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)

    Overlay histogram showing HeLa cells stained with ab208827 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208827, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 568 (ab209613) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / A375 (human malignant melanoma epithelial cell, Right) cells labelling SOX10 with Alexa Fluor® 568 Anti-SOX10 antibody [SP267] ab313897 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control: HeLa.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)

    Flow cytometry overlay histogram showing HepG2 cells stained with Alexa Fluor® 568 Anti-Ubiquitin antibody [EPR8830] ab307636 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (Alexa Fluor® 568 Anti-Ubiquitin antibody [EPR8830] ab307636) (1x 106 in 100μl at 1μg/ml (1/500)) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 568 (ab209613) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized B16-F0 (mouse melanoma epithelial-like cell) cells labelling SOX10 with Alexa Fluor® 568 Anti-SOX10 antibody [SP267] ab313897 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com