Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

• Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) THP-1 (human monocytic leukemia monocyte, Right) cells labelling CD300a with ab325696 at 1/50 dilution (1ug) / Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

• Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab325696 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

• Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab325696 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling MNDA with ab325039 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control.

Cells were stained with anti-CD19 conjugated to Alexa Fluor®647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling MNDA with ab325039 at 1/50000 dilution (0.001ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : K-562 (PMID : 12112016).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling MNDA with ab325039 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control.

Cells were stained with anti-CD56 conjugated to Brilliant Violet 421. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / A375 (human malignant melanoma epithelial cell, Right) cells labelling SOX10 with ab313897 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control : HeLa.

• Flow Cyt

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Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of L1CAM KO HeLa(human cervical adenocarcinoma epithelial cell, Left)

Parental HeLa(Right) cells labelling L1CAM with ab315361 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cell.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometry overlay histogram showing HepG2 cells stained with ab307636 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab307636) (1x 106 in 100μl at 1μg/ml (1/500)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 568 (ab209613) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.

• Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of Mouse bone marrow cells labelling Sialoadhesin/CD169 with ab316878 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control.

Mouse bone marrow cells are co-stained with F4/80 conjugated Alexa Fluor®647. Gated on viable cell.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized B16-F0 (mouse melanoma epithelial-like cell) cells labelling SOX10 with ab313897 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

• Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of Mouse splenocytes cells labelling PSGL-1 with ab323446 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells.
Cells are co-stained with CD3 conjugated to Alexa Fluor®647.

• Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Flow cytometric analysis of Mouse bone marrow cells labelling Sialoadhesin/CD169 with ab316878 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 568) (ab209613) / Left isotype control.

Mouse bone marrow cells are co-stained with CD3 conjugated Alexa Fluor®647. Gated on viable cell.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209613)

Overlay histogram showing HeLa cells stained with ab208827 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208827, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 568 (ab209613) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.