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Rabbit Monoclonal S100 beta antibody - conjugated to Alexa Fluor® 568. Astrocyte marker. Suitable for IHC-Fr and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB313817), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB313817), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB313817), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrICC/IF
Mouse
Tested
Not recommended
Rat
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

    1 products for Alternative Product

      Target data

      Function

      Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

      Alternative names

      Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

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      Rabbit Monoclonal S100 beta antibody - conjugated to Alexa Fluor® 568. Astrocyte marker. Suitable for IHC-Fr and reacts with Mouse, Rat samples.

      Alternative names

      Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

      Key facts

      Isotype

      IgG

      Conjugation

      Alexa Fluor® 568

      Excitation/Emission

      Ex: 578nm, Em: 603nm

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EP1576Y

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      3 product images

      • Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab313817), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab313817)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling S100 beta with ab313817 at 1/50 (10.0 µg/ml) (Green). Negative control: confocal image showing no staining on rat lung (PMID: 20844757). The nuclear counterstain was DAPI (Blue). The section was incubated with ab313817 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

      • Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab313817), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab313817)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling S100 beta with ab313817 at 1/50 (10.0 µg/ml) (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313817 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

      • Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab313817), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab313817)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling S100 beta with ab313817 at 1/50 (10.0 µg/ml) (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313817 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com