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AB324337

Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51]

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Rabbit Recombinant Monoclonal Sodium/Hydrogen Exchanger 1/NHE-1 antibody - conjugated to Alexa Fluor® 568. Suitable for IHC-P and reacts with Human, Mouse samples.

View Alternative Names

Nhe1, Sodium/hydrogen exchanger 1, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB324337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB324337)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab324337 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human liver.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB324337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB324337)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab324337 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human stomach.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB324337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB324337)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab324337 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on mouse liver.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB324337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] (AB324337)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab324337 at 1/50 (10.0 ug/ml) dilution.

Positive staining on mouse stomach.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Carrier free

    Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] - BSA and Azide free

  • Unconjugated

    Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28913-51

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen shares no sequence similarity with NHE-2, NHE-3, NHE-4, NHE-5. Based on the immunogen sequence, we do not expect this antibody to cross-react with the other family members. No cross-reactivity testing has been performed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (By similarity). Exchanges intracellular H(+) ions for extracellular Na(+) in 1 : 1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (By similarity).
See full target information Slc9a1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com