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AB312562

Alexa Fluor® 568 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

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Rabbit Recombinant Monoclonal Topoisomerase II beta/TOP2B antibody - conjugated to Alexa Fluor® 568.

View Alternative Names

DNA topoisomerase 2-beta, TOP2B

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • 578 PE

    PE Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • 660 APC

    APC Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • HRP

    HRP Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5377

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II alpha and Topoisomerase II beta also known as TOP2A and TOP2B are enzymes with a vital role in modulating the topological states of DNA. They actively introduce transient double-strand breaks in the DNA helix to manage DNA tangling and supercoiling during replication and transcription. TOP2A has a molecular weight of approximately 170 kilodaltons while TOP2B weighs around 180 kilodaltons. High levels of TOP2A are observed in proliferating cells such as those in the thymus and testis while TOP2B is expressed in nearly all tissues indicating its ubiquitous nature.
Biological function summary

These enzymes participate in fundamental cellular processes including chromosomal segregation replication and transcription. They function in the context of large protein complexes rather than in isolation. TOP2A is often engaged during mitosis due to its cell cycle-dependent expression. In contrast TOP2B's functions spread across various DNA-related actions such as differentiation and development. This distribution emphasizes their respective regulatory roles in both proliferating and resting cells.

Pathways

TOP2A and TOP2B integrate into critical pathways like the DNA replication and repair pathways. As a notable component of these pathways TOP2A interacts closely with proteins like MCM2-7 and ORC complex for replication origin unwinding while TOP2B shares interactions with factors like RNA polymerase II for transcription-related activities. These interactions reflect their involvement in maintaining genomic stability across different cellular conditions.

Aberrations in TOP2A expression or function link closely to numerous cancers reflecting its role in cell proliferation. Furthermore mutations or dysregulation of TOP2B have implications in disorders related to neural development highlighting its role beyond cellular growth control. Other related proteins like BRCA1 also display interactions with TOP2A reinforcing the enzyme's involvement in cancer-related pathways whereas TOP2B’s association with proteins like TAF(II)250 suggests a broader involvement in transcription processes relevant to neural disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. Plays a role in B-cell differentiation.
See full target information DNA topoisomerase 2-beta
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com