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AB325263

Alexa Fluor® 568 Anti-TPSB2 antibody [EPR28111-57]

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Rabbit Recombinant Monoclonal TPSB2 antibody - conjugated to Alexa Fluor® 568. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

TPS2, TPSB2, Tryptase beta-2, Tryptase-2, Tryptase II

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-TPSB2 antibody [EPR28111-57] (AB325263)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-TPSB2 antibody [EPR28111-57] (AB325263)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-937(human histiocytic lymphoma monocyte), K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling TPSB2 with ab325263 at 1/50 (10.0 ug/ml) dilution (green).

Confocal image showing cytoplasmic staining in U-937 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : K-562 (PMID : 8210998)
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (magenta).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28111-57

Isotype

IgG

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPSB2 also known as tryptase beta-2 is an enzymatic protein with substantial mechanical significance. It functions as a serine protease typically requiring activation by cleavage. The protein has an approximate mass of 30 kDa. TPSB2 is found mainly in mast cells and to a lesser extent in basophils suggesting its role in immune response. Expression of TPSB2 is not uniform across tissues reflecting its specialized functions.
Biological function summary

Tryptase beta-2 is involved in mediating allergic reactions and inflammation. It is part of a tetrameric complex that consists of identical subunits to perform its catalytic function efficiently. The activity of TPSB2 affects the degradation of extracellular matrix components during inflammatory responses. By modifying structural proteins TPSB2 can facilitate the passage of immune cells to sites of inflammation reinforcing its role in immune surveillance and defense.

Pathways

TPSB2 plays an important role within the immune response and inflammatory pathways. It is intricately connected to mast cell degranulation a process critical in initiating defense mechanisms. TPSB2 interacts with other proteins such as tryptase alpha/beta 1 (TPSAB1) to modulate cellular responses. Furthermore its presence in the kallikrein-kinin system emphasizes its role in modulating vascular inflammation and repair mechanisms.

TPSB2 has associations with allergic conditions like asthma and inflammatory diseases such as arthritis. Its activity influences the severity and progression of these conditions by promoting inflammatory processes. In asthma TPSB2 can contribute to airway remodeling through its interactions with other mast cell proteins like chymase augmenting inflammation and tissue changes. Understanding its mechanism in these diseases offers potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
See full target information TPSB2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com