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AB310366

Alexa Fluor® 594 Anti-ABCB11/BSEP antibody [EPR22670-64]

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Rabbit Recombinant Monoclonal ABCB11/BSEP antibody - conjugated to Alexa Fluor® 594.

View Alternative Names

BSEP, ABCB11, Bile salt export pump, ATP-binding cassette sub-family B member 11

  • 578 PE

    PE Anti-ABCB11/BSEP antibody [EPR22670-64]

  • 660 APC

    APC Anti-ABCB11/BSEP antibody [EPR22670-64]

  • HRP

    HRP Anti-ABCB11/BSEP antibody [EPR22670-64]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ABCB11/BSEP antibody [EPR22670-64]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ABCB11/BSEP antibody [EPR22670-64]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-ABCB11/BSEP antibody [EPR22670-64]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-ABCB11/BSEP antibody [EPR22670-64]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-ABCB11/BSEP antibody [EPR22670-64]

  • Unconjugated

    Anti-ABCB11/BSEP antibody [EPR22670-64]

  • Carrier free

    Anti-ABCB11/BSEP antibody [EPR22670-64] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22670-64

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCB11 also known as BSEP (bile salt export pump) is a protein that functions as an ATP-binding cassette transporter. It is encoded by the ABCB11 gene and has an approximate molecular mass of 160 kDa. BSEP is mainly expressed in the liver specifically on the canalicular membrane of hepatocytes. It facilitates the flow of bile acids from liver cells into the bile canaliculi playing an important role in bile acid homeostasis and detoxification processes within the liver.
Biological function summary

BSEP is essential for the transport of bile acids which are important components of bile necessary for the digestion and absorption of lipids and fat-soluble vitamins in the small intestine. BSEP functions as a homodimer and operates by using energy derived from ATP hydrolysis to export bile acids into bile. Malfunction of BSEP can impact bile flow and lead to severe liver conditions as it is the primary bile acid transporter at the canalicular membrane.

Pathways

BSEP's activity is integral to the enterohepatic circulation of bile acids. It works in conjunction with other transporters such as NTCP (sodium-taurocholate co-transporting polypeptide) and OSTα/β (organic solute transporter alpha-beta). These transporters play roles in the bile acid enterohepatic system contributing to liver-bile-gut circulation. Disruption in BSEP function can alter the FXR (farnesoid X receptor) signaling pathway critical in maintaining the balance of bile acid synthesis and metabolism.

Mutations or functional deficiencies in BSEP are linked to progressive familial intrahepatic cholestasis type 2 (PFIC2) and intrahepatic cholestasis of pregnancy (ICP). These disorders are characterized by impaired bile flow and accumulation of bile acids in the liver. BSEP dysfunction may also interact with other proteins such as MDR3 (multidrug resistance protein 3) which is involved in phospholipid transport and bile formation contributing further to liver pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed : 15791618, PubMed : 16332456, PubMed : 18985798, PubMed : 19228692, PubMed : 20010382, PubMed : 20398791, PubMed : 22262466, PubMed : 24711118, PubMed : 29507376, PubMed : 32203132). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (PubMed : 16332456). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (PubMed : 15901796, PubMed : 18245269).
See full target information ABCB11
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