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AB310544

Alexa Fluor® 594 Anti-AGL antibody [EPR8880]

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Rabbit Recombinant Monoclonal AGL/Alpha-glucosidase antibody - conjugated to Alexa Fluor® 594.

View Alternative Names

GDE, AGL, Glycogen debranching enzyme, Glycogen debrancher

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-AGL/Alpha-glucosidase antibody [EPR8880]

  • 578 PE

    PE Anti-AGL antibody [EPR8880]

  • 660 APC

    APC Anti-AGL antibody [EPR8880]

  • HRP

    HRP Anti-AGL antibody [EPR8880]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-AGL antibody [EPR8880]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-AGL antibody [EPR8880]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-AGL/Alpha-glucosidase antibody [EPR8880]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-AGL/Alpha-glucosidase antibody [EPR8880]

  • Unconjugated

    Anti-AGL/Alpha-glucosidase antibody [EPR8880]

  • Carrier free

    Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8880

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-glucosidase also known as AGL is an enzyme that breaks down carbohydrates by hydrolyzing alpha-14 glycosidic bonds in glycogen converting them into glucose molecules. This enzyme is important for the body's ability to derive glucose from stored glycogen a necessary process for maintaining energy levels. AGL is predominantly expressed in liver tissue where it plays an important role in glycogen metabolism. The AGL enzyme has an approximate mass of around 104 kDa. Additionally AGL can be referred to as alpha-16-glucosidase due to its specific hydrolytic capabilities on branching points in glycogen.
Biological function summary

AGL functions as part of the glycogen debranching enzyme complex which is important for glycogen catabolism. It cooperates with other enzymes to effectively mobilize glucose from glycogen stores. AGL's debranching activity ensures the smooth continuation of glycogenolysis providing glucose to meet energy demands during fasting or intense physical activity. This highlights the enzyme's importance in glucose homeostasis and metabolic processes that require rapid energy availability.

Pathways

AGL participates in the glycogenolysis and gluconeogenesis pathways. It partners with phosphorylase to release glucose-1-phosphate from glycogen ensuring energy supply during fasting conditions. The glycogenolysis pathway relies significantly on AGL for breaking down glycogen's branched structures while glucose-6-phosphatase assists in converting glucose-6-phosphate into free glucose. AGL's role in these pathways illustrates its importance in energy metabolism and glucose regulation.

AGL's dysfunction is linked to glycogen storage disease type III known as Cori disease. This genetic disorder results from mutations in the AGL gene causing a build-up of abnormally structured glycogen in the liver and muscles leading to symptoms such as hepatomegaly muscular weakness and hypoglycemia. AGL along with other enzymes involved in glycogen metabolism like phosphorylase kinase is a target for therapeutic interventions aimed at managing such glycogen storage diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional enzyme acting as 1,4-alpha-D-glucan : 1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
See full target information AGL
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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