Mouse Monoclonal alpha smooth muscle Actin antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Human samples. Cited in 14 publications.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-594-alpha-smooth-muscle-actin-antibody-1a4-ab202368--immunocytochemistry-immunofluorescence-img125968.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)")
Publications
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- EJNMMI research 13:672023Synthesis and preclinical evaluation of a novel fluorine-18 labeled small-molecule PET radiotracer for imaging of CXCR3 receptor in mouse models of atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSantosh R Alluri,Yusuke Higashi,Ashley Berendzen,Laurel A Grisanti,Lisa D Watkinson,Kamlendra Singh,Timothy J Hoffman,Terry Carmack,Elizabeth A Devanny,Miles Tanner,Kun-Eek KilPubMed 37438543
- Aging 15:4576-45992023Senescence and senotherapies in biliary atresia and biliary cirrhosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Jannone,Eliano Bonaccorsi Riani,Catherine de Magnée,Roberto Tambucci,Jonathan Evraerts,Joachim Ravau,Pamela Baldin,Caroline Bouzin,Axelle Loriot,Laurent Gatto,Anabelle Decottignies,Mustapha Najimi,Etienne Marc SokalPubMed 37204430
- Journal of the American Heart Association 12:e0274632023Diabetes Induces Cardiac Fibroblast Activation, Promoting a Matrix-Preserving Nonmyofibroblast Phenotype, Without Stimulating Pericyte to Fibroblast Conversion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinda Alex,Izabela Tuleta,Anis Hanna,Nikolaos G FrangogiannisPubMed 36892073
- Development (Cambridge, England) 149:2022Establishment and characterization of equine mammary organoids using a method translatable to other non-traditional model species.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArianna P Bartlett,Rebecca M Harman,Jennifer R Weiss,Gerlinde R Van de WallePubMed 35297994
- Kidney international 101:1017-10262022Digital spatial profiling of collapsing glomerulopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelly D Smith,David K Prince,Kammi J Henriksen,Roberto F Nicosia,Charles E Alpers,Shreeram AkileshPubMed 35227689
- International immunology 33:587-5942021Using a new three-dimensional CUBIC tissue-clearing method to examine the brain during experimental cerebral malaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Matsuo-Dapaah,Michelle Sue Jann Lee,Ken J Ishii,Kazuki Tainaka,Cevayir CobanPubMed 34455438
- International heart journal 62:381-3892021Therapeutic Efficacy of Cryopreserved, Allogeneic Extracellular Vesicles for Treatment of Acute Myocardial Infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer J Chung,Samuel T Kim,Samir Zaman,Mark R Helmers,Maria F Arisi,Elizabeth C Li,Zoe Tran,Carol W Chen,Peter Altshuler,Minna Chen,Jason A Burdick,Pavan AtluriPubMed 33731514
- Molecular therapy. Methods & clinical development 21:83-932021Alginate hydrogel polymers enable efficient delivery of a vascular-targeted AAV vector into aortic tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnca Remes,Dima Ibrahim Basha,Thomas Puehler,Christopher Borowski,Susanne Hille,Laura Kummer,Andreas H Wagner,Markus Hecker,Jasmin Soethoff,Georg Lutter,Derk Frank,Rawa Arif,Norbert Frey,Marcin Zaradzki,Oliver J MüllerPubMed 33768132
- The Journal of experimental medicine 216:1999-20092019A stromal cell niche sustains ILC2-mediated type-2 conditioning in adipose tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBatika M J Rana et. al.PubMed 31248899
- eLife 8:2019Programmed knockout mutation of liver fluke granulin attenuates virulence of infection-induced hepatobiliary morbidity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatpicha Arunsan et. al.PubMed 30644359
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Conjugation
Alexa Fluor® 594
- Excitation/Emission
Ex: 590nm, Em: 617nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cat | Predicted |
| Chicken | Predicted |
| Dog | Predicted |
| Goat | Predicted |
| Guinea pig | Predicted |
| Sheep | Predicted |
| Snake | Predicted |
| Zebrafish | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes This product gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). ab178001 - Mouse monoclonal IgG2a (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Version
6 products for Alternative Product
Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
ACTSA, ACTVS, GIG46, ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Recommended products
Mouse Monoclonal alpha smooth muscle Actin antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Human samples. Cited in 14 publications.
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Conjugation
Alexa Fluor® 594
- Excitation/Emission
Ex: 590nm, Em: 617nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Clone number
1A4
- Purification technique
Affinity purification Protein G
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
- Biological function summary
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
- Pathways
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
- Associated diseases and disorders
Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-594-alpha-smooth-muscle-actin-antibody-1a4-ab202368--immunocytochemistry-immunofluorescence-img125968.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
ab202368 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202368 at 1/200 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 100% methanol (5min).
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