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AB202368

Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4]

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(27 Publications)

Anti-alpha smooth muscle Actin antibody [1A4] - Alexa Fluor® 594 conjugated (ab202368) is a mouse monoclonal antibody detecting alpha smooth muscle Actin in ICC/IF. Suitable for Human.

- Over 10 publications

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

4 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)

ab202368 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202368 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 100% methanol (5min).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling alpha smooth muscle Actin with ab202368 at 1/50 dilution (Magenta).

Confocal image showing cytoplasmic staining in HeLa cell line (shown in magenta). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (green).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SV40LT-SMC (rat SV40 transfected aortic smooth muscle cell) cells labelling alpha smooth muscle Actin with ab202368 at 1/50 dilution (Magenta).

Confocal image showing cytoplasmic staining in SV40LT-SMC cell line (shown in magenta). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (green).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (AB202368)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling alpha smooth muscle Actin with ab202368 at 1/50 dilution (Magenta).

Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in magenta). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (green).

  • HRP

    HRP Anti-alpha smooth muscle Actin antibody [1A4]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]

  • 519 FITC

    FITC Anti-alpha smooth muscle Actin antibody [1A4]

  • Biotin

    Biotin Anti-alpha smooth muscle Actin antibody [1A4]

  • Unconjugated

    Anti-alpha smooth muscle Actin antibody [1A4]

  • Carrier free

    Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1A4

Isotype

IgG2a

Light chain type

kappa

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p>This product gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p><p>ab178001 - Mouse monoclonal IgG2a (Alexa Fluor<sup>®</sup> 594), is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Cat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Snake": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368) is a mouse monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-alpha smooth muscle Actin [1A4] Alexa Fluor® 594 (ab202368) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.

Related products
Antibody clone 1A4 is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha smooth muscle Actin Alexa Fluor® 594 [1A4] (ab202368).

Other related products
We have a range of other formats of antibody clone [1A4] also available for your convenience: ab7817, FITC - ab8211, Biotin - ab125057, Alexa Fluor® 488 - ab184675, Alexa Fluor® 594 - ab202368, HRP - ab203696, Carrier free - ab240654

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
See full target information ACTA2
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111790 PubMed39935459

2025

Spatial transcriptomic analysis identifies epithelium-macrophage crosstalk in endometriotic lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory W Burns,Zhen Fu,Erin L Vegter,Zachary B Madaj,Erin Greaves,Idhaliz Flores,Asgerally T Fazleabas

The veterinary quarterly 45:1-14 PubMed39921381

2025

The secretomes of bovine mammary epithelial cell subpopulations differentially modulate macrophage function.

Applications

Unspecified application

Species

Unspecified reactive species

Nikola Danev,Rebecca M Harman,Anja S Sipka,Leanne Oliveira,Lucas Huntimer,Gerlinde R Van de Walle

Bone research 13:12 PubMed39837840

2025

FGFR antagonists restore defective mandibular bone repair in a mouse model of osteochondrodysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Morice,Amélie de La Seiglière,Alexia Kany,Roman H Khonsari,Morad Bensidhoum,Maria-Emilia Puig-Lombardi,Laurence Legeai Mallet

Frontiers in immunology 15:1475051 PubMed39737187

2024

CRP deposition in human abdominal aortic aneurysm is associated with transcriptome alterations toward aneurysmal pathogenesis: insights from spatial whole transcriptomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Na Kim,Hee Young Seok,Joon Seo Lim,Jiwon Koh,Jeong Mo Bae,Chong Jai Kim,Ga-Hyeon Ryu,You Jung Ok,Jae-Sung Choi,Chung-Hyun Cho,Se Jin Oh

Stem cell research & therapy 15:429 PubMed39543714

2024

Immortalized mammosphere-derived epithelial cells retain a bioactive secretome with antimicrobial, regenerative, and immunomodulatory properties.

Applications

Unspecified application

Species

Unspecified reactive species

Nikola Danev,Julia M Poggi,Emilie A Dewever,Arianna P Bartlett,Leane Oliveira,Lucas Huntimer,Rebecca M Harman,Gerlinde R Van de Walle

Gut 74:364-386 PubMed39438124

2024

IQGAP3 signalling mediates intratumoral functional heterogeneity to enhance malignant growth.

Applications

Unspecified application

Species

Unspecified reactive species

Mitsuhiro Shimura,Junichi Matsuo,ShuChin Pang,Nawaphat Jangphattananont,Aashiq Hussain,Muhammad Bakhait Rahmat,Jung-Won Lee,Daisuke Douchi,Jasmine Jie Lin Tong,Khine Myint,Supriya Srivastava,Ming Teh,Vivien Koh,Wei Peng Yong,Jimmy Bok Yan So,Patrick Tan,Khay-Guan Yeoh,Michiaki Unno,Linda Shyue Huey Chuang,Yoshiaki Ito

American journal of physiology. Renal physiology 327:F91-F102 PubMed38721662

2024

Validation of an organ mapping antibody panel for cyclical immunofluorescence microscopy on normal human kidneys.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Brewer,Lukasz G Migas,Kelly A Clouthier,Jamie L Allen,David M Anderson,Ellie Pingry,Melissa Farrow,Ellen M Quardokus,Jeffrey M Spraggins,Raf Van de Plas,Mark P de Caestecker

Journal of neuroinflammation 21:105 PubMed38649885

2024

Pathological high intraocular pressure induces glial cell reactive proliferation contributing to neuroinflammation of the blood-retinal barrier via the NOX2/ET-1 axis-controlled ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Shi,Panpan Li,Marc Herb,Hanhan Liu,Maoren Wang,Xiaosha Wang,Yuan Feng,Tim van Beers,Ning Xia,Huige Li,Verena Prokosch

Advanced healthcare materials 13:e2302831 PubMed38394389

2024

A 3D Bioprinted Human Neurovascular Unit Model of Glioblastoma Tumor Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yen-Ting Tung,Yu-Chi Chen,Kristy Derr,Kelli Wilson,Min Jae Song,Marc Ferrer

Cell 186:3706-3725.e29 PubMed37562402

2023

Distinct molecular profiles of skull bone marrow in health and neurological disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynep Ilgin Kolabas,Louis B Kuemmerle,Robert Perneczky,Benjamin Förstera,Selin Ulukaya,Mayar Ali,Saketh Kapoor,Laura M Bartos,Maren Büttner,Ozum Sehnaz Caliskan,Zhouyi Rong,Hongcheng Mai,Luciano Höher,Denise Jeridi,Muge Molbay,Igor Khalin,Ioannis K Deligiannis,Moritz Negwer,Kenny Roberts,Alba Simats,Olga Carofiglio,Mihail I Todorov,Izabela Horvath,Furkan Ozturk,Selina Hummel,Gloria Biechele,Artem Zatcepin,Marcus Unterrainer,Johannes Gnörich,Jay Roodselaar,Joshua Shrouder,Pardis Khosravani,Benjamin Tast,Lisa Richter,Laura Díaz-Marugán,Doris Kaltenecker,Laurin Lux,Ying Chen,Shan Zhao,Boris-Stephan Rauchmann,Michael Sterr,Ines Kunze,Karen Stanic,Vanessa W Y Kan,Simon Besson-Girard,Sabrina Katzdobler,Carla Palleis,Julia Schädler,Johannes C Paetzold,Sabine Liebscher,Anja E Hauser,Ozgun Gokce,Heiko Lickert,Hanno Steinke,Corinne Benakis,Christian Braun,Celia P Martinez-Jimenez,Katharina Buerger,Nathalie L Albert,Günter Höglinger,Johannes Levin,Christian Haass,Anna Kopczak,Martin Dichgans,Joachim Havla,Tania Kümpfel,Martin Kerschensteiner,Martina Schifferer,Mikael Simons,Arthur Liesz,Natalie Krahmer,Omer A Bayraktar,Nicolai Franzmeier,Nikolaus Plesnila,Suheda Erener,Victor G Puelles,Claire Delbridge,Harsharan Singh Bhatia,Farida Hellal,Markus Elsner,Ingo Bechmann,Benjamin Ondruschka,Matthias Brendel,Fabian J Theis,Ali Erturk
View all publications
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