Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker

41 product images

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized FUS KO HAP1 (FUS knockout human chronic myelogenous leukemia near-haploid cell) cells labelling TLS/FUS with Anti-TLS/FUS antibody [P3-A6C4] ab323832 at 1/1000 (1.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

  Confocal image showing nuclear staining in parental HAP1 cell line (shown in green) and no staining in FUS KO HAP1 cells. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry analysis of SK-OV-3 (human ovarian cancer epithelial cell)cells labelling Zic2 with Anti-Zic2 antibody [EPR7790] ab150404 at 2.5 μg/ml. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry analysis of Jurkat (human T cell leukemia T lymphocyte) labeling p95/NBS1 with purified Anti-p95/NBS1 (phospho S343) antibody [EP178] ab109453 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.27 μg/ml) was used as counterstain. Nuclei were stained blue with DAPI. Negative control: PBS instead of the primary antibody.

  Confocal image showing increased nuclear staining in Jurkat cells treated with Etoposide (25uM) for 2 h.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of HeLa (Human cervix adenocarcinoma epithelial cell) using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 25 ug/mL anisomycin for 30 min, then Lambda Protein Phosphatase 31 for 2 hours cells labeling Hsp27 with purified Anti-Hsp27 (phospho S78) antibody [Y175] ab32501 at 1/50 dilution (11.3 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) dilution. Goat anti-rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of HeLa cells using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling KDM3B / JMJD1B with Anti-KDM3B / JMJD1B antibody [EPR24219-30] ab271046 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubμue Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-937 cells labelling IL-8 with Anti-IL-8 antibody [EPR26511-74] ab289967 at 1/100 (5.87 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic staining is observed in U-937 cells treated with TPA (100 ng/mL) for 24 h, then LPS (5 μg/mL) for 7 h with Brefeldin A (300 ng/mL) for the last 3 h. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of NIH/3T3 cells using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling KDM3B / JMJD1B with Anti-KDM3B / JMJD1B antibody [EPR24219-30] ab271046 at 1/500 dilution, followed by 150077 Goat Anti-Rabbit IgG H&L (Alexa Fluo^r® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
  

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  ab195889 staining alpha Tubulin in MEF1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% PBS-Triton X-100 for 5 min then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1hr. The cells were then incubated with ab195889 at 1/250 dilution (shown in orange) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
  

  This product gave a positive signal in 100% methanol (10 min) fixed MEF1 cells under the same testing conditions.

  Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC cells labelling S100A8+S100A9 with Anti-S100A8 + S100A9 antibody [RM1038] ab288715 at 1/500 (0.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in subsets of mouse PBMCs is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of SK-N-BE(2) cells using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-N-BE(2) cells labelling MAP2 with Anti-MAP2 antibody [RM1037] - Neuronal Marker ab288714 at 1/500 (0.942 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in SK-N-BE(2) cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  ab195889 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% PBS-Triton X-100 for 5 min then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1hr. The cells were then incubated with ab195889 at 1/167 dilution (shown in psedo-color red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

  This product gave a positive signal in 100% methanol (10 min) fixed HeLa cells under the same testing conditions.

  Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized LAMP1 KO HAP1 cells labelling LAMP1 with Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker ab278043 at 1/100 (5.45 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (green). Confocal image showing cytoplasmic staining in parental HAP1 cell line and no staining in LAMP1 KO HAP1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody- Microtubule Marker (Alexa Fluor ^® 594) was used to counterstain at 1/200 (red).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of A431 (Human epidermoid carcinoma epithelial cell) cells using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling S100 alpha 2 using Anti-S100 alpha 2/S100A2 antibody [EPR5392] ab109494. The cells were fixed with 100% Methanol then permeabilized with 0.1% Triton X-100. The cells were then incubated with Anti-S100 alpha 2/S100A2 antibody [EPR5392] ab109494 at 1:100 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of Jurkat (Human T cell leukemia T lymphocyte) cells using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling NDRG1 using Anti-NDRG1 antibody [EPR5593] ab124689. The cells were fixed with 100% Methanol then permeabilized with 0.1% Triton X-100. The cells were then incubated with Anti-NDRG1 antibody [EPR5593] ab124689 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells using mouse Anti-alpha Tubulin antibody

  Immunocytochemical/immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (human glioblastoma- astrocytoma epithelial cell) cells labelling AKR1C1/AKR1C2 with primary antibody anti-AKR1C1/AKR1C2 (Anti-AKR1C1/AKR1C2 antibody [EPR11542] ab179448) at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in U-87 MG cell line. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1:200 dilution. The nuclear counter stain is DAPI (blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of A431 (Human epidermoid carcinoma epithelial cell) cells using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling FRA1 using Anti-FRA1 antibody [EP4711] ab124722. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with Anti-FRA1 antibody [EP4711] ab124722 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of HeLa (Human cervix adenocarcinoma epithelial cell) using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PKC beta 2 using Anti-PKC beta 2 antibody [Y125] ab32026. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with Anti-PKC beta 2 antibody [Y125] ab32026 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of Human PBMC (Human primary peripheral blood mononuclear cell) cells using mouse Anti-alpha Tubulin antibody

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of ES-D3 (mouse blastocyst-derived embryonic stem cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ES-D3 (mouse blastocyst-derived embryonic stem cell) cells labelling KLF5 with Anti-KLF5 antibody [EPR29066-77] ab324105 at 1/100 (4.87 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing nuclear staining in ES-D3 [D3] cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of PC-12 (rat adrenal gland pheochromocytoma cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Thrombospondin 1 with Anti-Thrombospondin 1 antibody [EPR22927-54] ab267388 at 1/100 (5.3 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor_® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor_® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor_® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of MCF7 (human breast adenocarcinoma epithelial cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling RNF20 with Anti-RNF20 antibody [EPR13562(B)] ab181104 at 1/200 (8.5 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing nuclear staining in MCF7 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) labeling BMAL1 with Anti-BMAL1 antibody [EPR23696-22] ab230822 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 was used as a counterstain.

  The nuclear counterstain is DAPI (blue).

  Confocal image showing positive staining in PC-3 cell line.

  Negative Control: Raji (PMID: 29230015).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of SW480 (Human colorectal adenocarcinoma epithelial cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma epithelial cell) labeling 15-PGDH with Anti-15-PGDH antibody [EPR14332] ab187160 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 was used as a counterstain (red).

  The nuclear counterstain is DAPI (blue).

  Confocal image showing mainly cytoplasmic staining in SW480 cell line.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of MCF-7 (human breast adenocarcinoma epithelial cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast adenocarcinoma epithelial cell) labeling 15-PGDH with Anti-15-PGDH antibody [EPR14332] ab187160 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 was used as a counterstain (red).

  The nuclear counterstain is DAPI (blue).

  Confocal image showing mainly cytoplasmic staining in MCF-7 cell line.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of HepG2 (human hepatocellular carcinoma epithelial cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) labelling ROR beta/RORB with Anti-ROR beta/RORB antibody [EPR15552] - N-terminal ab187657 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution was used as a counterstain (red).

  The nuclear counterstain is DAPI (blue).

  Confocal image showing nuclear staining in HepG2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) labeling FABP5 with Anti-FABP5 antibody [EPR22552-64] ab255276 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 was used as a counterstain (red).

  The nuclear counterstain is DAPI (blue).

  Confocal image showing cytoplasmic and nuclear staining in MDA-MB-231 cells.

  Negative Control: T-47D (PMID: 21356353).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of HUVEC (human umbilical vein endothelial cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 100% methanol fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial) cells labelling IL-33 with Anti-IL-33 antibody [EPR26367-2] ab316846 at 1/500 dilution, followed by Goat anti-Rabbit (AlexaFluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution. Confocal image showing the nuclear and weak cytoplasmic staining (shown in green) is decreased after the treatment with IL-1β (5 ng/ml) for 24 hours in HUVEC cells. Nuclear DNA was labeled with DAPI (shown in blue).

  Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A]-Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (shown in magenta).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  The reduction in IL-33 when stimulated with TNFa in HUVEC cells (specially the nuclear IL-33) (PMID: 18787100).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of CLDN1 KO A431(CLDN1 knockout human epidermoid carcinoma epithelial cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CLDN1 KO A431(CLDN1 knockout human epidermoid carcinoma epithelial cell) cells labelling Claudin 1 with Anti-Claudin 1 antibody [EPR25359-48] ab307692 at 1/250 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) antibody at 1/1000 dilution (Green).

  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^®
  594) ( ab195889 ) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is AlexaFluor488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  Confocal image showing membranous and cytoplasmic staining in wildtype A431 cells and showing no staining in CLDN1 knockout A431 cells.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of HeLa (Human cervix adenocarcinoma epithelial cell) cells using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling IMP3 using Anti-IMP3 antibody [EPR12021] ab177477. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with Anti-IMP3 antibody [EPR12021] ab177477 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of rat splenocytes using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat splenocytes labelling GDAP1 with Anti-GDAP1 antibody [EPR29581-48] ab323844 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Low expression: Confocal image showing no staining in rat splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of PBMC (Human primary peripheral blood mononuclear cell) cells using mouse Anti-alpha Tubulin antibody

  Immunocytochemistry/ Immunofluorescence analysis of PBMC (Human primary peripheral blood mononuclear cell) cells labeling IL-2 using Anti-IL-2 antibody [EPR2780] ab92381. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with Anti-IL-2 antibody [EPR2780] ab92381 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling C3 with Anti-C3 antibody [RM1270] ab324288 at 1/500 (1.052 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in HepG2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: A-172.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of mouse splenocytes using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes labelling GDAP1 with Anti-GDAP1 antibody [EPR29581-48] ab323844 at 1/100 (5.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Low expression: Confocal image showing no staining in mouse splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab195889 200 2.5ug/ml dilution (Magenta).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of Human PBMC using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC cells labelling MNDA with Alexa Fluor® 488 Anti-MNDA antibody [EPR28267-44] ab324034 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in subsets of human PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1(human monocytic leukemia monocyte) cells labelling MNDA with Alexa Fluor® 488 Anti-MNDA antibody [EPR28267-44] ab324034 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Negative control: K-562 (PMID: 12112016).
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of COLO 201 (human derived from metastatic site ascites bipolar, slightly refractile, fibroblast-like cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized COLO 201 (human derived from metastatic site ascites bipolar, slightly refractile, fibroblast-like cell) cells labelling Lyn (phospho Y397) with Anti-Lyn (phospho Y397) antibody [EPR26354-1] ab300118 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) antibody at 1/1000 dilution (Green).

  Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (ab195889)  was used to counterstain tubulin at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of A431 (Human epidermoid carcinoma epithelial cell) cells using mouse Anti-alpha Tubulin antibody

  Anti-Collagen XVII antibody [EPR14758] ab186415 staining Collagen XVII in A431 (Human epidermoid carcinoma epithelial cell) cells. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% tritonX-100. The cells were then incubated with Anti-Collagen XVII antibody [EPR14758] ab186415 at 10μg/ml concentration followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used at 1/200 dilution as counterstain (shown in red). Secondary antibody only control: PBS instead of the primary antibody.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling p15 INK4b with Anti-p15 INK4b antibody [EPR29010-111] ab323851 at 1/100 (5.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing increased cytoplasmic staining in MCF7 cells (shown in green) treated with TGFβ (10 ng/mL) for 18 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution.