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AB310525

Alexa Fluor® 594 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

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Rabbit Recombinant Monoclonal FABP-1 antibody - conjugated to Alexa Fluor® 594. Suitable for Target Binding Affinity, Antibody Labelling, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm, cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • HRP

    HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 578 PE

    PE Anti-Aspartate Aminotransferase +FABP-1 antibody [EPR12145]

  • 660 APC

    APC Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • Unconjugated

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

  • Carrier free

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12145

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, Flow Cyt (Intra), Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as glutamate oxaloacetate transaminase is an enzyme that plays an important role in amino acid metabolism. AST transfers an amino group typically from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. This enzyme approximately weighs 45 kDa. AST is widely expressed in tissues with especially high levels found in the liver heart skeletal muscle and kidneys.
Biological function summary

Aspartate aminotransferase participates in the malate-aspartate shuttle which facilitates the transfer of reducing equivalents across the mitochondrial membrane. This shuttle system is important in maintaining the balance of NADH and NAD+ between the cytosol and mitochondria. AST forms part of a protein complex that includes α-ketoglutarate and oxaloacetate which are central to its transaminase activity in cellular metabolism.

Pathways

Aspartate aminotransferase is significantly involved in the tricarboxylic acid (TCA) cycle and the urea cycle. Within the TCA cycle AST ensures the linkage between carbohydrate and amino acid metabolism by converting aspartate and α-ketoglutarate into oxaloacetate and glutamate. In the urea cycle the enzyme connects to argininosuccinate synthetase and argininosuccinate lyase facilitating the removal of excess nitrogen from the body.

Elevated levels of aspartate aminotransferase are often indicative of liver damage including conditions like hepatitis and cirrhosis. Additionally the enzyme associates with myocardial infarction due to its high activity in cardiac tissue. Liver-related disorders may also involve fatty acid-binding protein 1 (FABP-1) which closely interacts with AST and serves as a marker for liver function and damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
See full target information GOT2

Additional targets

Aspartate aminotransferase, cytoplasmic
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com