Skip to main content

Rabbit Recombinant Monoclonal ATP6V1D antibody - conjugated to Alexa Fluor® 594.

Be the first to review this product! Submit a review

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (PubMed:21844891).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ATP6V1D antibody - conjugated to Alexa Fluor® 594.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR11326(B)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ATP6V1D also known as V1D is a subunit of the V1 domain of vacuolar ATPase (V-ATPase). This enzyme is essential for catalyzing ATP hydrolysis and is mitochondrial proton pump playing roles in acidifying various cell compartments. With a molecular weight of approximately 36 kDa ATP6V1D is expressed in many tissues with higher levels noted in the kidney liver and brain. It helps regulate the pH of intracellular compartments important for cellular processes like protein degradation and neurotransmitter uptake.

Biological function summary

ATP6V1D is an integral part of the V-ATPase complex. The V-ATPases have a critical role in the acidification of eukaryotic intracellular compartments which are important for processes such as protein sorting zymogen activation and receptor-mediated endocytosis. This complex operates in various cell types and facilitates the transport of protons across membranes maintaining the acidic environments required for enzyme activity and molecular processing.

Pathways

ATP6V1D participates in key cellular functions such as the Wnt signaling pathway and endocytosis. In the Wnt pathway it contributes to regulating cellular growth and differentiation and interacts with other proteins like ATP6V1A and ATP6V1F to sustain vesicular trafficking and pH homeostasis. These interactions support the movement and function of vesicles affecting the fusion of endosomes and lysosomes essential for cellular responses to external stimuli.

Associated diseases and disorders

Abnormalities involving ATP6V1D have been linked to neurodegenerative diseases and renal tubular acidosis. Researchers have observed that malfunction of V-ATPases including the ATP6V1D subunit can alter pH regulation in neurons associated with Alzheimer's disease. Furthermore ATP6V1D interactions with proteins such as ATP6V1C1 in the context of impaired proton pumping contribute to metabolic acidosis affecting kidney function and leading to conditions like Type I renal tubular acidosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com