Rabbit Recombinant Monoclonal ATP6V1D antibody - conjugated to Alexa Fluor® 594.
IgG
Rabbit
Alexa Fluor® 594
Ex: 590nm, Em: 617nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
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Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
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Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (PubMed:21844891).
ATP6M, VATD, VATD, ATP6V1D, ATP6M, V-type proton ATPase subunit D, V-ATPase subunit D, V-ATPase 28 kDa accessory protein, Vacuolar proton pump subunit D
Rabbit Recombinant Monoclonal ATP6V1D antibody - conjugated to Alexa Fluor® 594.
IgG
Rabbit
Alexa Fluor® 594
Ex: 590nm, Em: 617nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR11326(B)
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This supplementary information is collated from multiple sources and compiled automatically.
ATP6V1D also known as V1D is a subunit of the V1 domain of vacuolar ATPase (V-ATPase). This enzyme is essential for catalyzing ATP hydrolysis and is mitochondrial proton pump playing roles in acidifying various cell compartments. With a molecular weight of approximately 36 kDa ATP6V1D is expressed in many tissues with higher levels noted in the kidney liver and brain. It helps regulate the pH of intracellular compartments important for cellular processes like protein degradation and neurotransmitter uptake.
ATP6V1D is an integral part of the V-ATPase complex. The V-ATPases have a critical role in the acidification of eukaryotic intracellular compartments which are important for processes such as protein sorting zymogen activation and receptor-mediated endocytosis. This complex operates in various cell types and facilitates the transport of protons across membranes maintaining the acidic environments required for enzyme activity and molecular processing.
ATP6V1D participates in key cellular functions such as the Wnt signaling pathway and endocytosis. In the Wnt pathway it contributes to regulating cellular growth and differentiation and interacts with other proteins like ATP6V1A and ATP6V1F to sustain vesicular trafficking and pH homeostasis. These interactions support the movement and function of vesicles affecting the fusion of endosomes and lysosomes essential for cellular responses to external stimuli.
Abnormalities involving ATP6V1D have been linked to neurodegenerative diseases and renal tubular acidosis. Researchers have observed that malfunction of V-ATPases including the ATP6V1D subunit can alter pH regulation in neurons associated with Alzheimer's disease. Furthermore ATP6V1D interactions with proteins such as ATP6V1C1 in the context of impaired proton pumping contribute to metabolic acidosis affecting kidney function and leading to conditions like Type I renal tubular acidosis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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