AB201771

Alexa Fluor® 594 Anti-beta Catenin antibody [E247]

RabMAb
Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(30 Publications)

Rabbit Recombinant Monoclonal beta Catenin antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 30 publications.

View Alternative Names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Catenin antibody [E247] (AB201771)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Catenin antibody [E247] (AB201771)

ab201771 staining beta Catenin in Caco-2 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201771 at 1/100 dilution (pseudocolored red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 2μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-beta Catenin antibody [E247] (AB201771)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-beta Catenin antibody [E247] (AB201771)

Overlay histogram showing HAP1 wildtype (green line) and HAP1-CNNB1 knockout cells (red line) stained with ab201771. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab201771, 0.1μg/ml) for 30 min at 22°C.

A mouse IgG1 isotype control antibody (ab208568) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CNNB1 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.

This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min) permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-beta Catenin antibody [E247]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-beta Catenin antibody [E247]
HRP

HRP Anti-beta Catenin antibody [E247]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-beta Catenin antibody [E247]
Unconjugated

Anti-beta Catenin antibody [E247] - ChIP Grade
Carrier free

Anti-beta Catenin antibody [E247] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E247

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.1 µg/mL", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.

Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).

See full target information CTNNB1

style

left-column

Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Experimental biology and medicine (Maywood, N.J.) 240:45-57 PubMed25053781

2014

Featured article: induction of heme oxygenase with hemin improves pericardial adipocyte morphology and function in obese Zucker rats by enhancing proteins of regeneration.

Applications

Unspecified application

Species

Rat

Joseph Fomusi Ndisang,Shuchita Tiwari

International journal of medical sciences 11:971-8 PubMed25076852

2014

Wnt signaling as a possible promoting factor of cell differentiation in pleomorphic adenomas.

Applications

IHC-P

Species

Human

Yukiko Okuda,Keisuke Nakano,Koji Suzuki,Yoshihiko Sugita,Katsutoshi Kubo,Hatsuhiko Maeda,Norimasa Okafuji,Hiromasa Hasegawa,Toshiyuki Kawakami

PloS one 9:e99137 PubMed24918446

2014

Dose-dependent effect of estrogen suppresses the osteo-adipogenic transdifferentiation of osteoblasts via canonical Wnt signaling pathway.

Applications

Unspecified application

Species

Mouse

Bo Gao,Qiang Huang,Yan-Shui Lin,Bo-Yuan Wei,Yun-Shan Guo,Zhen Sun,Long Wang,Jing Fan,Hong-Yang Zhang,Yue-Hu Han,Xiao-Jie Li,Jun Shi,Jian Liu,Liu Yang,Zhuo-Jing Luo

Oncology letters 8:85-90 PubMed24959224

2014

Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell progression via activation of the non-canonical Wnt signaling pathway.

Applications

Species

Unspecified reactive species

Ya Li Shen,Qing Luo,Yu Xia Guo,Gai Huai Zheng,Jie Yu,You Hua Xu

PloS one 9:e93364 PubMed24806734

2014

Wnt/β-catenin pathway regulates cementogenic differentiation of adipose tissue-deprived stem cells in dental follicle cell-conditioned medium.

Applications

ICC/IF, WB

Species

Rat, Rat

Na Liu,Bin Gu,Ning Liu,Xin Nie,Bo Zhang,Xia Zhou,Manjing Deng

PloS one 9:e91086 PubMed24755605

2014

Wnt signaling in form deprivation myopia of the mice retina.

Applications

Species

Mouse

Mingming Ma,Zhengwei Zhang,Ergang Du,Wenjing Zheng,Qing Gu,Xun Xu,Bilian Ke

Disease models & mechanisms 7:693-700 PubMed24652769

2014

Cryptosporidium parvum-induced ileo-caecal adenocarcinoma and Wnt signaling in a mouse model.

Applications

ICC/IF

Species

Mouse

Sadia Benamrouz,Valerie Conseil,Magali Chabé,Marleen Praet,Christophe Audebert,Renaud Blervaque,Karine Guyot,Sophie Gazzola,Anthony Mouray,Thierry Chassat,Baptiste Delaire,Nathalie Goetinck,Nausicaa Gantois,Marwan Osman,Christian Slomianny,Vanessa Dehennaut,Tony Lefebvre,Eric Viscogliosi,Claude Cuvelier,Eduardo Dei-Cas,Colette Creusy,Gabriela Certad

Blood cancer journal 4:e192 PubMed24632884

2014

Deregulated WNT signaling in childhood T-cell acute lymphoblastic leukemia.

Applications

Species

Human

O H Ng,Y Erbilgin,S Firtina,T Celkan,Z Karakas,G Aydogan,E Turkkan,Y Yildirmak,C Timur,E Zengin,J J M van Dongen,F J T Staal,U Ozbek,M Sayitoglu

Biology of reproduction 90:48 PubMed24451984

2014

Loss of Sertoli-germ cell adhesion determines the rapid germ cell elimination during the seasonal regression of the seminiferous epithelium of the large hairy armadillo Chaetophractus villosus.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Pablo Luaces,Luis Francisco Rossi,Roberta Beatriz Sciurano,Paola Rebuzzini,Valeria Merico,Maurizio Zuccotti,Maria Susana Merani,Silvia Garagna

Oncogenesis 2:e89 PubMed24566388

2014

The essential role of TNIK gene amplification in gastric cancer growth.

Applications

IHC

Species

Human

D-H Yu,X Zhang,H Wang,L Zhang,H Chen,M Hu,Z Dong,G Zhu,Z Qian,J Fan,X Su,Y Xu,L Zheng,H Dong,X Yin,Q Ji,J Ji

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-beta Catenin antibody [E247]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Catenin antibody [E247] (AB201771)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-beta Catenin antibody [E247] (AB201771)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise