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Rabbit Recombinant Monoclonal Beta-5-tubulin antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (AB206369), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 594
Excitation/Emission
Ex: 590nm, Em: 617nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted
Chicken
Predicted
Cow
Predicted
Dog
Predicted
Drosophila melanogaster
Predicted
Monkey
Predicted
Xenopus tropicalis
Predicted
Zebrafish
Predicted

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Chicken, Cow, Dog, Drosophila melanogaster, Monkey, Zebrafish, Xenopus tropicalis
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

Recommended products

Rabbit Recombinant Monoclonal Beta-5-tubulin antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 594
Excitation/Emission
Ex: 590nm, Em: 617nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16774
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Associated diseases and disorders

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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15 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    ab206369 staining beta Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206369 at 1/100 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369)

    Beta Tubulin Immunocytochemistry/ Immunofluorescence staining of C2C12 (mouse myoblast) using rabbit Anti-beta Tubulin antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling SIRT1 with Anti-SIRT1 antibody [19A7AB4] ab110304 at 1/100 dilution followed by secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used as a counterstain at a 1/200 dilution.

    Confocal image showing nuclear staining in C2C12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369)

    Beta Tubulin Immunocytochemistry/ Immunofluorescence staining of PC-12 (rat adrenal gland pheochromocytoma cell) using rabbit Anti-beta Tubulin antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling SIRT1 with Anti-SIRT1 antibody [19A7AB4] ab110304 at 1/100 dilution followed by secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used as a counterstain at a 1/200 dilution.

    Confocal image showing nuclear staining in PC-12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369)

    Beta Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta Tubulin antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling CD40L with Anti-CD40L antibody [AT161-10] ab281905 at 1/100 dilution followed by secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/200 dilution.

    ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used as a counterstain at a 1/1000 dilution.

    Confocal image showing membranous staining in subsets of human PBMCs treated with Phorbol-12-myristate-13-acetate (50 ng/mL) and Ionomycin (500 ng/mL) for 6 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369)

    Beta Tubulin Immunocytochemistry/ Immunofluorescence staining of MOLT-4 treated with 10ng/ml PMA and 500ng/ml Ionomycin using rabbit Anti-beta Tubulin antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling PD1 with Anti-PD1 antibody [NAT105] ab52587 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution at RT for 45 min. Recombinant Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369) was used as a counterstain at 1/50 dilution and was co-incubated with Anti-PD1 antibody [NAT105] ab52587 overnight at 4° C. Nucleus were visualized using DAPI. Confocal image showing membranous staining in MOLT-4 cells treated with 500 ng/ml Ionomycin and 10 ng/ml Phorbol-12-myristate-13-acetate (PMA) for 24 hours, and no staining in MOLT-4 cells.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labelling Lewis a,b carbohydrate with Anti-Lewis a,b carbohydrate antibody [HEA164] ab322960 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing cytoplasmic and membranous staining in BxPC-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Negative control: HepG2.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling P cadherin with Anti-P cadherin antibody [56/P-Cadherin] ab307740 at 1/100 dilution (8.94 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).

    Confocal image showing membranous staining in A431 cell line, no staining was observed in MCF7 cell line.

    Negative control: MCF7 (PMID:10545506).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized Saos-2 (human osteosarcoma epithelial cell) cells labelling NPAT with Anti-NPAT antibody [27/NPAT] ab307837 at 1/250 dilution (3.99 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).

    Confocal image showing nuclear foci staining in Saos-2 cell line.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling NPAT with Anti-NPAT antibody [27/NPAT] ab307837 at 1/250 dilution (3.99 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2ug/ml) (Green).

    Confocal image showing nuclear foci staining in A431 cell line.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (10ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2ug/ml).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling NPAT with Anti-NPAT antibody [27/NPAT] ab307837 at 1/250 dilution (3.99 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2ug/ml) (Green).

    Confocal image showing nuclear foci staining in U-2 OS cell line. The number of positive-staining cells decreased after treatment with 20 uM roscovitine for 48 h (PMID: 14976556).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (5ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2ug/ml).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized HDLM-2 (human Hodgkin lymphoma cell) cells labelling Semaphorin 4D/CD100 with Anti-Semaphorin 4D/CD100 antibody [30/CD100] ab307685 at 1/100 dilution (9.97 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).

    Confocal image showing membranous staining in HDLM-2 cell line, no staining was observed in PC-3 cell line.

    Negative control: PC-3 (PMID: 23775445).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling MHC Class II with Anti-MHC Class II antibody [MRC OX-6] ab23990 at 1/100 dilution (10.63 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used at 1/100 dilution (5µg/mL) as counterstain for tubulin (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody.

    Confocal image showing membranous and cytoplasmic staining in subsets of mouse splenocyte . Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (ab206369)

    Beta Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta Tubulin antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SIRT1 KO A549 (SIRT1 knockout human lung carcinoma epithelial cell) cells labelling SIRT1 with Anti-SIRT1 antibody [19A7AB4] ab110304 at 1/100 dilution followed by secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used as a counterstain at a 1/200 dilution.

    Confocal image showing nuclear staining in parental A549 cell line and no staining in SIRT1 KO A549 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat splenocyte cells labelling MHC Class II with Anti-MHC Class II antibody [MRC OX-6] ab23990 at 1/100 dilution (10.63 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used at 1/100 dilution (5µg/mL) as counterstain for tubulin (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody.

    Confocal image showing membranous and cytoplasmic staining in subsets of rat splenocyte.
    Negative control: C6.
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

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