AB206369

Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Beta-5-tubulin antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

24 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2ug/ml) (Green). Confocal image showing nuclear foci staining in U-2 OS cell line. The number of positive-staining cells decreased after treatment with 20 uM roscovitine for 48 h (PMID : 14976556). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (5ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2ug/ml) (Green). Confocal image showing nuclear foci staining in A431 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (10ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labelling Lewis a,b carbohydrate with ab322960 at 1/2000 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic and membranous staining in BxPC-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : HepG2.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized HDLM-2 (human Hodgkin lymphoma cell) cells labelling Semaphorin 4D/CD100 with ab307685 at 1/100 dilution (9.97 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing membranous staining in HDLM-2 cell line, no staining was observed in PC-3 cell line. Negative control : PC-3 (PMID : 23775445). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling P cadherin with ab307740 at 1/100 dilution (8.94 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing membranous staining in A431 cell line, no staining was observed in MCF7 cell line. Negative control : MCF7 (PMID : 10545506). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CBX1 KO HAP1(human chronic myelogenous leukemia cell)/Wild-type HAP1 cells labelling CBX1 / HP1 beta with ab10811 at 1/100 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution(Green).

Confocal image showing nuclear staining in parental HAP1 cell line (shown in green) and negative staining in CBX1 KO HAP1 cell line. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SIRT1 KO A549 (SIRT1 knockout human lung carcinoma epithelial cell) cells labelling SIRT1 with ab110304 at 1/100 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used as a counterstain at a 1/200 dilution.

Confocal image showing nuclear staining in parental A549 cell line and no staining in SIRT1 KO A549 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling CD40L with ab281905 at 1/100 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/200 dilution.

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used as a counterstain at a 1/1000 dilution.

Confocal image showing membranous staining in subsets of human PBMCs treated with Phorbol-12-myristate-13-acetate (50 ng/mL) and Ionomycin (500 ng/mL) for 6 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

ab206369 staining beta Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206369 at 1/100 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells with ab5408 at 1/500 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used for counterstain at a 1/100 dilution (Red). Nuclear DNA was labelled with DAPI (shown in blue)

Confocal image showing nuclear staining in HeLa cell line, the signal decreased after phosphatase treatment at 37°C for 2h.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling PD1 with ab52587 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) at 1/1000 dilution at RT for 45 min. Recombinant Alexa Fluor^® 594 Anti-beta Tubulin antibody [EPR16774] (ab206369) was used as a counterstain at 1/50 dilution and was co-incubated with ab52587 overnight at 4° C. Nucleus were visualized using DAPI. Confocal image showing membranous staining in MOLT-4 cells treated with 500 ng/ml Ionomycin and 10 ng/ml Phorbol-12-myristate-13-acetate (PMA) for 24 hours, and no staining in MOLT-4 cells.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte cell) cells labelling MNDA with ab188566 at 1/200 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing nuclear staining in THP-1 cell line (shown in green). Negative control : HeLa.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain tubulin at a 1/500 dilution (Red).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized Saos-2 (human osteosarcoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing nuclear foci staining in Saos-2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing membranous staining in J774A.1 cell line (shown in green), no staining was observed in C2C12 cell line. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control : C2C12.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling SIRT1 with ab110304 at 1/100 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used as a counterstain at a 1/200 dilution.

Confocal image showing nuclear staining in C2C12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling SIRT1 with ab110304 at 1/100 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used as a counterstain at a 1/200 dilution.

Confocal image showing nuclear staining in PC-12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CBX1 / HP1 beta with ab10811 at 1/100 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution(Green).

Confocal image showing nuclear staining in NIH/3T3 cell line (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells with ab5408 at 1/500 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used for counterstain at a 1/100 dilution (Red). Nuclear DNA was labelled with DAPI (shown in blue)

Confocal image showing nuclear staining in NIH/3T3 cell line, the signal decreased after phosphatase treatment at 37°C for 2h.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells with ab117579 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used for counterstain at a 1/200 dilution (Magenta).

Confocal image showing membranous and cytoplasmic staining in subsets of mouse PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling MHC Class II with ab23990 at 1/100 dilution (10.63 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used at 1/100 dilution (5µg/mL) as counterstain for tubulin (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody. Confocal image showing membranous and cytoplasmic staining in subsets of mouse splenocyte . Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat splenocyte cells labelling MHC Class II with ab23990 at 1/100 dilution (10.63 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used at 1/100 dilution (5µg/mL) as counterstain for tubulin (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody. Confocal image showing membranous and cytoplasmic staining in subsets of rat splenocyte. Negative control : C6. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing cytoplasmic staining in mouse splenocytes (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing membranous staining in WEHI-231 cell line (shown in green), no staining was observed in Neuro-2a cell line. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control : Neuro-2a.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker
HRP

HRP Anti-beta Tubulin antibody [EPR16774] - Loading Control
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker
660 APC

APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker
Unconjugated

Anti-beta Tubulin antibody [EPR16774] - Loading Control
Carrier free

Anti-beta Tubulin antibody [EPR16774] - BSA and Azide free (Capture)
Carrier free

Anti-beta Tubulin antibody [EPR16774] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16774

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Drosophila melanogaster": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 11:101079 PubMed38560501

2024

CYP3A5 unexpectedly regulates glucose metabolism through the AKT-TXNIP-GLUT1 axis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Shao,Qingfei Pan,Haiyan Tan,Jing Wu,Ha Won Lee,Andrew D Huber,William C Wright,Ji-Hoon Cho,Jiyang Yu,Junmin Peng,Taosheng Chen

JOR spine 2:e1049 PubMed31463463

2019

Aquaporin expression in the human and canine intervertebral disc during maturation and degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph W Snuggs,Rebecca E Day,Frances C Bach,Matthew T Conner,Rowena A D Bunning,Marianna A Tryfonidou,Christine L Le Maitre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB206369)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise