AB203439

Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal Calnexin antibody - conjugated to Alexa Fluor® 594. Endoplasmic Reticulum Membrane marker. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.

View Alternative Names

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Calnexin with ab203439 at 1/50 dilution.

Positive staining on human gastric cancer.
The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Calnexin with ab203439 at 1/50 dilution.

Positive staining on human pancreas.
The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

Overlay histogram showing HeLa cells stained with ab203439 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min.

The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab203439, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 610/20 bandpass filter.

HRP

HRP Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
660 APC

APC Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
578 PE

PE Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
Unconjugated

Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker
Carrier free

Anti-Calnexin antibody [EPR3633(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3633(2)

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 45:7827-7841 PubMed37886937

2023

T-Lymphocytes Activated by Dendritic Cells Loaded by Tumor-Derived Vesicles Decrease Viability of Melanoma Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Yurevich Filin,Yuriy Pavlovich Mayasin,Chulpan Bulatovna Kharisova,Anna Valerevna Gorodilova,Daria Sergeevna Chulpanova,Kristina Viktorovna Kitaeva,Albert Anatolyevich Rizvanov,Valeria Vladimirovna Solovyeva

FEBS open bio 14:740-755 PubMed37199081

2023

Endothelial cell-derived extracellular vesicles induce pro-angiogenic responses in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hüseyin Abdik,Oğuz Kaan Kırbaş,Batuhan Turhan Bozkurt,Ezgi Avşar Abdik,Taha Bartu Hayal,Fikrettin Şahin,Pakize Neslihan Taşlı

Journal of orthopaedic translation 39:147-162 PubMed37188001

2023

Forkhead box O3 attenuates osteoarthritis by suppressing ferroptosis through inactivation of NF-κB/MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhao,Guantong Sun,Yaxin Li,Keyu Kong,Xiaodong Li,Tianyou Kan,Fei Yang,Lei Wang,Xiaoqing Wang

Current issues in molecular biology 45:571-592 PubMed36661524

2023

Cytochalasin B-Induced Membrane Vesicles from TRAIL-Overexpressing Mesenchymal Stem Cells Induce Extrinsic Pathway of Apoptosis in Breast Cancer Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Daria S Chulpanova,Tamara V Pukhalskaia,Zarema E Gilazieva,Yuliya V Filina,Milana N Mansurova,Albert A Rizvanov,Valeriya V Solovyeva

Current issues in molecular biology 44:5363-5378 PubMed36354675

2022

Comparative Analysis of Natural and Cytochalasin B-Induced Membrane Vesicles from Tumor Cells and Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zarema Gilazieva,Daria Chulpanova,Aleksei Ponomarev,Ivan Filin,Ekaterina Garanina,Albert Rizvanov,Valeriya Solovyeva

Frontiers in immunology 13:824378 PubMed35401544

2022

Preclinical Studies on Convalescent Human Immune Plasma-Derived Exosome: Omics and Antiviral Properties to SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Neslihan Pakize Taşlı,Zeynep Burçin Gönen,Oğuz Kaan Kırbaş,Nur Seda Gökdemir,Batuhan Turhan Bozkurt,Buse Bayrakcı,Derya Sağraç,Ezgi Taşkan,Sevda Demir,Nur Ekimci Gürcan,Melike Bayındır Bilgiç,Ömer Faruk Bayrak,Hazel Yetişkin,Büşra Kaplan,Shaikh Terkıs Islam Pavel,Gökçen Dinç,Müge Serhatlı,Gamze Çakırca,Ahmet Eken,Vedat Aslan,Mehmet Yay,Musa Karakukcu,Ekrem Unal,Fethi Gül,Kemal Erdem Basaran,Yusuf Ozkul,Fikrettin Şahin,Olcay Y Jones,Şaban Tekin,Aykut Özdarendeli,Mustafa Cetin

BioNanoScience 12:293-301 PubMed35261871

2022

Analysis of the Interaction of Human Neuroblastoma Cell-Derived Cytochalasin B Induced Membrane Vesicles with Mesenchymal Stem Cells Using Imaging Flow Cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Valeriya V Solovyeva,Kristina V Kitaeva,Daria S Chulpanova,Svetlana S Arkhipova,Ivan Yu Filin,Albert A Rizvanov

Scientific reports 9:19159 PubMed31844310

2019

Optimized Isolation of Extracellular Vesicles From Various Organic Sources Using Aqueous Two-Phase System.

Applications

Unspecified application

Species

Unspecified reactive species

Oğuz Kaan Kırbaş,Batuhan Turhan Bozkurt,Ayla Burçin Asutay,Beyza Mat,Bihter Ozdemir,Dilek Öztürkoğlu,Hülya Ölmez,Zeynep İşlek,Fikrettin Şahin,Pakize Neslihan Taşlı

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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