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AB208073

Alexa Fluor® 594 Anti-Cathepsin L/V/K/H antibody [EPR8011]

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(1 Publication)

Rabbit Recombinant Monoclonal Cathepsin L/MEP antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CTSL1, CTSL, Procathepsin L, Cathepsin L1, Major excreted protein, MEP

1 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Cathepsin L/V/K/H antibody [EPR8011] (AB208073)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Cathepsin L/V/K/H antibody [EPR8011] (AB208073)

Overlay histogram showing A549 cells stained with ab208073 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208073, 1/500 dilution) for 30 min at 22°C.Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • 578 PE

    PE Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • 660 APC

    APC Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • HRP

    HRP Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • Unconjugated

    Anti-Cathepsin L/V/K/H antibody [EPR8011]

  • Carrier free

    Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8011

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsins L V K and H are essential cysteine proteases that serve important functions in protein catabolism. These proteases participate in proteolytic processes by digesting proteins into shorter peptides and amino acids. Cathepsin L also known as CTSL typically has a molecular mass of about 25-35 kDa and is expressed in various tissues such as the liver spleen and placenta. Cathepsin K plays a similar role in bone resorption and is highly expressed in osteoclasts. Cathepsin V and Cathepsin H meanwhile have distinct tissue distributions with cathepsin V often found in the thymus and testis whereas cathepsin H's expression spans across different cell types contributing a vast array of biological functions.
Biological function summary

Cathepsins regulate cellular homeostasis and modulate various physiological processes. These proteases perform their functions within the lysosomal compartment although they can also participate in extracellular matrix remodeling and other non-lysosomal roles. Cathepsin K for example is part of the cathepsin protein family and aids in degrading collagen therefore playing a pivotal role in bone turnover and resorption. Cathepsins can act independently or in some cases are part of larger enzyme complexes enhancing their proteolytic activity to handle different substrates driven by the physiological demand.

Pathways

Cathepsins key in the lysosomal degradation pathway and influence other signaling cascades like the apoptotic pathway. The regulation of these pathways often involves interactions with other proteins such as pro-apoptotic members of the Bcl-2 family facilitating cell death under certain conditions. Cathepsin L contributes to the antigen processing pathway linking it to the immune response. Cathepsin K on the other hand acts closely with structural proteins like collagen in the bone metabolism pathway highlighting its significance in skeletal development and maintenance.

Altered cathepsin activity closely associates with conditions like osteoporosis and cancer. Cathepsin K upregulation can lead to excessive bone degradation seen in osteoporosis while aberrant cathepsin L expression relates to cancer progression and metastasis due to its role in degrading extracellular matrix components facilitating tumor invasion. In osteoporosis interactions with matrix metalloproteinases further enhance bone matrix breakdown exacerbating disease symptoms. In cancer the relationship between cathepsin L and the urokinase-type plasminogen activator system highlights its role in promoting malignancy and suggests potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (By similarity). In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter (By similarity). In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells (By similarity). Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation (By similarity). Secreted form generates endostatin from COL18A1 (PubMed : 10716919). Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation (By similarity). Required for maximal stimulation of steroidogenesis by TIMP1 (By similarity).. (Microbial infection) In cells lacking TMPRSS2 expression, facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via a slow acid-activated route with the proteolysis of coronavirus spike (S) glycoproteins in lysosome for entry into host cell (PubMed : 16339146, PubMed : 18562523, PubMed : 32142651, PubMed : 32221306, PubMed : 37990007). Proteolysis within lysosomes is sufficient to activate membrane fusion by coronaviruses SARS-CoV and EMC (HCoV-EMC) S as well as Zaire ebolavirus glycoproteins (PubMed : 16081529, PubMed : 18562523, PubMed : 26953343).. Isoform 2. Functions in the regulation of cell cycle progression through proteolytic processing of the CUX1 transcription factor (PubMed : 15099520). Translation initiation at downstream start sites allows the synthesis of isoforms that are devoid of a signal peptide and localize to the nucleus where they cleave the CUX1 transcription factor and modify its DNA binding properties (PubMed : 15099520).
See full target information CTSL

Additional targets

CTSK,CTSV,CTSH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:2024 PubMed29789628

2018

Targetable vulnerabilities in T- and NK-cell lymphomas identified through preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Y Ng,Noriaki Yoshida,Amanda L Christie,Mahmoud Ghandi,Neekesh V Dharia,Joshua Dempster,Mark Murakami,Kay Shigemori,Sara N Morrow,Alexandria Van Scoyk,Nicolas A Cordero,Kristen E Stevenson,Maneka Puligandla,Brian Haas,Christopher Lo,Robin Meyers,Galen Gao,Andrew Cherniack,Abner Louissaint,Valentina Nardi,Aaron R Thorner,Henry Long,Xintao Qiu,Elizabeth A Morgan,David M Dorfman,Danilo Fiore,Julie Jang,Alan L Epstein,Ahmet Dogan,Yanming Zhang,Steven M Horwitz,Eric D Jacobsen,Solimar Santiago,Jian-Guo Ren,Vincent Guerlavais,D Allen Annis,Manuel Aivado,Mansoor N Saleh,Amitkumar Mehta,Aviad Tsherniak,David Root,Francisca Vazquez,William C Hahn,Giorgio Inghirami,Jon C Aster,David M Weinstock,Raphael Koch
View all publications
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Product promise

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