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AB286808

Alexa Fluor® 594 Anti-CCR2 antibody [EPR20844]

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Rabbit Recombinant Monoclonal CCR2 antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD192, Cmkbr2, Ccr2, C-C chemokine receptor type 2, C-C CKR-2, CC-CKR-2, CCR-2, CCR2, JE/FIC receptor, MCP-1 receptor

1 Images
Flow Cytometry - Alexa Fluor® 594 Anti-CCR2 antibody [EPR20844] (AB286808)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 594 Anti-CCR2 antibody [EPR20844] (AB286808)

Flow cytometric analsysi of C57 BL/6 mouse splenocytes with ab286808 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 μ/ml anti CD16/CD32 and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab286808) or Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) isotype (1x 106 in 100μl at 0.04 μg/ml (1/12500)) for 30 min on ice. The cells were simultaneously stained with Ly6G.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter. Events were gated on live single cells.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CCR2 antibody [EPR20844]

  • 660 APC

    APC Anti-CCR2 antibody [EPR20844]

  • 578 PE

    PE Anti-CCR2 antibody [EPR20844]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CCR2 antibody [EPR20844]

  • Unconjugated

    Anti-CCR2 antibody [EPR20844]

  • Carrier free

    Anti-CCR2 antibody [EPR20844] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20844

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR2 also known as C-C chemokine receptor type 2 is a protein involved in immune cell trafficking. It is a member of the G-protein-coupled receptor family and has a molecular mass of approximately 43 kDa. CCR2 is primarily expressed on monocytes dendritic cells and certain subsets of T cells. It functions as a receptor for monocyte chemoattractant proteins (MCPs) with MCP-1 (CCL2) being the most well-known ligand. The interaction between CCR2 and its ligands directs the movement of these immune cells to sites of inflammation or tissue injury.
Biological function summary

CCR2 plays an important role in mediating leukocyte migration. It acts in the immune system to guide monocytes from the bloodstream into tissues contributing to immune surveillance and response. CCR2 operates not as part of a larger receptor complex but it does interact closely with other chemokine receptors which may influence its signaling. The receptor's activity has critical implications for inflammatory processes and lies at the heart of many immune responses.

Pathways

CCR2 is integrally involved in the chemokine signaling pathway and the inflammatory response pathway. Its function in these pathways highlights its role in modulating immune cell infiltration during immune challenges. The receptor also interfaces with other important signaling proteins such as CCR5 which like CCR2 is another chemokine receptor involved in mediating immune cell movement. These interactions overlap and complement each other offering nuanced regulation of immune cell dynamics.

CCR2 has connections to conditions such as atherosclerosis and rheumatoid arthritis. In atherosclerosis the receptor's involvement in monocyte recruitment to the arterial wall is an important step in plaque formation. Its role in rheumatoid arthritis centers on the promotion of leukocyte infiltration into the joint tissues contributing to inflammation and joint damage. CCR2's connection to such disorders often aligns with a similar role played by other chemokine receptors like CCR5 highlighting its relevance in inflammation-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key functional receptor for CCL2 but can also bind CCL7 and CCL12 chemokines (PubMed : 8631787, PubMed : 8662823, PubMed : 8996246). Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (PubMed : 28507030). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (PubMed : 29930553). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed : 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed : 29993042). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (PubMed : 24806994, PubMed : 29632244).
See full target information Ccr2
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Product promise

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