Alexa Fluor® 594 Anti-CD34 antibody [EPR27431-71]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD34 antibody [EPR27431-71] (AB324151)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling CD34 with ab324151 at 1/50 (10.0 ug/ml) dilution.

Positive staining on basement membrane of mouse testis.
The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD34 antibody [EPR27431-71] (AB324151)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD34 with ab324151 at 1/50 (10.0 ug/ml) dilution.

Low expression : nearly no staining on mouse liver.
The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD34 antibody [EPR27431-71] (AB324151)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling CD34 with ab324151 at 1/50 (10.0 ug/ml) dilution.

Positive staining on endothelial cells of mouse pancreas.
The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Anti-CD34 antibody [EPR27431-71] (AB324151)

Flow cytometric analysis of Mouse bone marrow cells labelling CD34 with ab324151 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

Gated on viable cells.
Cells are co-stained with CD117 conjugated to APC-eFluor 780. (PMID : 23295606)

• Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Anti-CD34 antibody [EPR27431-71] (AB324151)

Flow cytometric analysis of Neuro-2a (mouse neuroblastoma neuroblast, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling CD34 with ab324151 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : Neuro-2a.
Gated on viable cells.