Alexa Fluor® 594 Anti-CNPase antibody [11-5B]
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(1 Publication)
Mouse Monoclonal CNPase antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Rat samples. Cited in 1 publication.
View Alternative Names
CNP, CNPase
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-CNPase antibody [11-5B] (AB320064)
ab320064 staining CNPase in primary hippocampal rat neurons/glia, DIV14 cells (obtained from Neuromics, cat. no. PC35101). The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab320064 at 1 ug/ml (shown in magenta). ab195887, mouse monoclonal to alpha Tubulin (Alexa Fluor® 488) (shown in green) was used at 2 ug/ml for structural counterstaining.
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This product also work with 100% methanol (5 min) fixation under the same testing conditions.
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HRP Anti-CNPase antibody [11-5B]
Reactivity data
Product details
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Publications (1)
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IUBMB life 73:784-799 PubMed33715270
2021
Applications
Unspecified application
Species
Unspecified reactive species
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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