Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y]

• IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203443)

Immunohistochemical analysis of Paraffin-embedded Human pancreas tissue labelling Cytokeratin 19 with ab203443 at 1/100 dilution.

Membrane staining on human pancreas.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203443)

Immunohistochemical analysis of Paraffin-embedded Human lung cancer tissue labelling Cytokeratin 19 with ab203443 at 1/100 dilution.

Membrane staining on human lung cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203443)

Immunohistochemical analysis of Paraffin-embedded Human glioma tissue labelling Cytokeratin 19 with ab203443 at 1/100 dilution.

Negative control : no staining on human glioma.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203443)

ab203443 staining Cytokeratin 19 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203443 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (AB203443)

ab203443 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab203443 at 1/500 dilution (shown in red) and ab195887 (Mouse monoclonal to alpha Tubulin - Alexa Fluor® 488) at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).