AB203419

Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Desmin antibody - conjugated to Alexa Fluor® 594. Cytoskeleton marker. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Desmin, DES

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (AB203419)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (AB203419)

ab203419 staining Desmin in A673 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203419 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in A673 cells fixed with 100% methanol (5 min).

Unconjugated

Anti-Desmin antibody [Y66] - Cytoskeleton Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Desmin antibody [Y66] - Cytoskeleton Marker
660 APC

APC Anti-Desmin antibody [Y66] - Cytoskeleton Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Desmin antibody [Y66] - Cytoskeleton Marker
578 PE

PE Anti-Desmin antibody [Y66] - Cytoskeleton Marker
HRP

HRP Anti-Desmin antibody [Y66] - Cytoskeleton Marker
Carrier free

Anti-Desmin antibody [Y66] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Desmin antibody [Y66] - Cytoskeleton Marker
Biotin

Biotin Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y66

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

ab32362 reacts with an epitope located in the C terminal region of desmin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in A673 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmin also referred to as Desmin 20 is an intermediate filament protein with a molecular weight of approximately 53 kDa. Primarily found in muscle cells desmin provides structural integrity to cells being an important component of the cytoskeleton. Desmin expression is highly specific to cardiac skeletal and smooth muscle tissues and it can be detected using desmin marker techniques like desmin IHC (Immunohistochemistry). Desmin staining or desmin stain methods are commonly employed in diagnostic labs to visualize this protein within tissue samples.

Biological function summary

Desmin plays a significant role in maintaining cellular architecture by anchoring organelles such as nuclei and mitochondria within the cytoplasm. It associates with other proteins to form desmin intermediate filament networks which facilitate force transmission and maintain mechanical integrity. This protein does not operate in isolation but is part of a larger complex of cytoskeletal elements that reinforce muscle cell shape and resilience. Its interactions and binding with other cytoskeletal components confirm its essential role in muscle structure.

Pathways

Desmin links closely to the cell structure and function pathways providing stability during muscle contraction processes. It interacts with other proteins including actin and tubulin within the cytoskeleton network ensuring coordinated responses to mechanical stress. Specifically desmin integrates into pathways related to the maintenance of the sarcomere structure thereby connecting to additional important proteins like myosin and dystrophin which collectively uphold muscular function and integrity.

Desmin mutations can lead to myopathies such as desmin-related myopathy (DRM) or cardiomyopathy where the structural disruptions result in abnormal muscle function. These conditions typically manifest as muscle weakness or cardiac abnormalities. Furthermore its relationship to the sarcomere suggests interactions with proteins like dystrophin in muscular dystrophies where desmin integrity critically influences disease progression and severity. Understanding desmin's link to such disorders underpins its role as a diagnostic marker and a potential therapeutic target in muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed : 25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed : 24200904, PubMed : 25394388, PubMed : 26724190). May act as a sarcomeric microtubule-anchoring protein : specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

See full target information DES

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70025 PubMed40520075

2025

Adipose-derived stem cell exosomes alleviate TGF-β1-induced urethral stricture fibrosis by suppressing the TGF-β/Smad pathway and downstream PDGFR-β/RAS/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liang,Chao Deng,Hang Guo,Zhenghao Dai,Yiwen Jiang,Yuting Lu,Weiguo Chen

Cancers 13: PubMed34199452

2021

HGF/c-Met Inhibition as Adjuvant Therapy Improves Outcomes in an Orthotopic Mouse Model of Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tony C Y Pang,Zhihong Xu,Alpha Raj Mekapogu,Srinivasa Pothula,Therese Becker,Susan Corley,Marc R Wilkins,David Goldstein,Romano Pirola,Jeremy Wilson,Minoti Apte

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (AB203419)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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