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AB206913

Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

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(3 Publications)

Rabbit Recombinant Monoclonal EEA1 antibody - conjugated to Alexa Fluor® 594. Early Endosome marker. Suitable for ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB206913)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB206913)

ab206913 staining EEA1 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206913 at 1/1000 dilution (shown in orange). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

  • Unconjugated

    Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

  • Carrier free

    Anti-EEA1 antibody [EPR4245] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4245

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)</p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.
Biological function summary

EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.

Pathways

EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.

EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
See full target information EEA1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 8:4736-4746 PubMed36777573

2023

Tween-20-Modified BiVO Nanorods for CT Imaging-Guided Radiotherapy of Tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Peng,Yifan Hao,Chao Si,Bo Wang,Chengfeng Luo,Menghao Chen,Cheng Luo,Baijuan Gong,Zhimin Li

Proceedings of the National Academy of Sciences of the United States of America 119:e2110491119 PubMed35294288

2022

Replication is the key barrier during the dual-host adaptation of mosquito-borne flaviviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Zhang,Dening Liang,Fei Yuan,Yiran Yan,Zuoshu Wang,Pan Liu,Qi Yu,Xing Zhang,Xiangxi Wang,Aihua Zheng

Journal of virology 93: PubMed31167919

2019

and Inhibition of the Infectivity of Human Enterovirus 71 by a Sulfonated Food Azo Dye, Brilliant Black BN.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Meng,Qiang Jia,Sek-Man Wong,Kaw-Bing Chua
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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