Rabbit Recombinant Monoclonal eEF1A1/EF-Tu antibody - conjugated to Alexa Fluor® 594.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Select an associated product type
Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed:26593721, PubMed:26651998, PubMed:36123449, PubMed:36264623, PubMed:36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed:26593721, PubMed:26651998, PubMed:36123449, PubMed:36264623, PubMed:36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed:26593721, PubMed:26651998, PubMed:36123449, PubMed:36264623). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed:17177976). (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.
EEF1A1P5
EEF1A, EF1A, LENG7, EEF1A1, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Leukocyte receptor cluster member 7, EF-Tu, eEF1A-1
Rabbit Recombinant Monoclonal eEF1A1/EF-Tu antibody - conjugated to Alexa Fluor® 594.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
The immunogen used for this product shares 6 continuous identical amino acids with eEF1A2. Cross-reactivity with this protein has not been confirmed experimentally.
EE1AL3/EEF1A1P5 it's a suspected pseudogene (https://www.ncbi.nlm.nih.gov/gene/158078). Our validation shows that this clone is able to detect Recombinant EF1AL3/EEF1A1P5 but endogenous protein expression is yet unknown.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The eEF1A1 also known as EF-Tu or eEF1A1-L3 is an elongation factor involved in protein synthesis. It facilitates the delivery of aminoacyl-tRNA to the A-site of the ribosome during translation. eEF1A1 exhibits a mass of approximately 50 kDa and is widely expressed in various tissues including the brain heart and skeletal muscle. Its expression level varies indicating that it plays a consistent role in translation machinery but may have tissue-specific functions.
This elongation factor is important in promoting the accuracy and efficiency of protein translation. It interacts with several components of the protein synthesis machinery and forms part of a larger translational complex. eEF1A1 is involved in ensuring the correct amino acids are added during protein synthesis which affects protein structure and function. In addition to its canonical role it also engages in processes such as actin filament binding nuclear transport and other cell maintenance tasks.
The activities of eEF1A1 intersect with key processes involved in cellular growth and proliferation. It plays a significant role in the mTOR signaling pathway which regulates cell growth by controlling protein synthesis. eEF1A1 also interacts with proteins like Rheb an important player in mTOR signaling. By participating in these pathways eEF1A1 links translation with broader regulatory networks that control cell metabolism.
EEF1A1 has connections to cancer and neurodegenerative diseases. Its elevated expression is often observed in several cancer types suggesting a role in tumorigenesis. Additionally misregulation of eEF1A1 may contribute to neurodegenerative disorders such as Alzheimer's disease where alterations in protein synthesis pathways are common. The protein's interaction with tau known in neuropathological conditions highlights its impact on neurodegeneration.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com