AB202227

Alexa Fluor® 594 Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NSE antibody - conjugated to Alexa Fluor® 594. Neuron marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2, ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE, Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB202227)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB202227)

ab202227 staining ENO1 + ENO2 + ENO3 in SKNSH cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202227 at 1/200 dilution (pseudocolored in red) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 2μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker
578 PE

PE Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker
Unconjugated

Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker
Carrier free

Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3377

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.

Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (By similarity). Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

See full target information ENO2

Additional targets

ENO1,ENO3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18075 PubMed38213100

2024

C-X-C chemokine receptor type 4 promotes tubular cell senescence and renal fibrosis through β-catenin-inhibited fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Qinyu Wu,Qiurong Chen,Dan Xu,Xiaoxu Wang,Huiyun Ye,Xiaolong Li,Yabing Xiong,Jiemei Li,Shan Zhou,Jinhua Miao,Weiwei Shen,Youhua Liu,Hongxin Niu,Ying Tang,Lili Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB202227)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Proteins & Peptides

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise