Alexa Fluor® 594 Anti-Fibronectin antibody [F1]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Fibronectin antibody [F1] (AB275336)

ab275336 staining Fibronectin in HepG2 (human hepatocellular carcinoma epithelial cell) cells at 1/100 dilution (5 μg/ml). The cells were fixed with 4% Paraformaldehyde, and permeabilized with 0.1% PBS-Triton X-100. Confocal image showing positive cytoplasmic staining in HepG2 cell line (shown in magenta). The counterstain, ab7291 mouse anti-Tubulin at 1/1000 (1 μg/ml) was observed in green. ab150113 Fluor®488 Goat anti- Mouse secondary at 1/1000 (2 μg/ml) was used to detect the counterstain. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Negative control：293T

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Fibronectin antibody [F1] (AB275336)

Immunofluorescence staining of Fibronectin staining in a section of formalin-fixed paraffin-embedded human breast carcinoma*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110°C for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab275336 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.