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AB217225

Alexa Fluor® 594 Anti-G3BP antibody [EPR13986(B)]

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(5 Publications)

Rabbit Recombinant Monoclonal G3BP antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

G3BP, G3BP1, Ras GTPase-activating protein-binding protein 1, G3BP-1, ATP-dependent DNA helicase VIII, GAP SH3 domain-binding protein 1, hDH VIII

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-G3BP antibody [EPR13986(B)] (AB217225)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-G3BP antibody [EPR13986(B)] (AB217225)

ab217225 staining G3BP in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab217225 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-G3BP antibody [EPR13986(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-G3BP antibody [EPR13986(B)]

  • 578 PE

    PE Anti-G3BP antibody [EPR13986(B)]

  • 660 APC

    APC Anti-G3BP antibody [EPR13986(B)]

  • HRP

    HRP Anti-G3BP antibody [EPR13986(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-G3BP antibody [EPR13986(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-G3BP antibody [EPR13986(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-G3BP antibody [EPR13986(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13986(B)

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein involved in various processes, such as stress granule formation and innate immunity (PubMed : 12642610, PubMed : 20180778, PubMed : 23279204, PubMed : 30510222, PubMed : 30804210). Plays an essential role in stress granule formation (PubMed : 12642610, PubMed : 20180778, PubMed : 23279204, PubMed : 32302570, PubMed : 32302571, PubMed : 32302572, PubMed : 34739333, PubMed : 35977029, PubMed : 36183834, PubMed : 36279435, PubMed : 36692217, PubMed : 37379838). Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress (PubMed : 12642610, PubMed : 20180778, PubMed : 23279204, PubMed : 27022092, PubMed : 32302570, PubMed : 32302571, PubMed : 32302572, PubMed : 36279435, PubMed : 37379838). Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding : functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations (PubMed : 32302570, PubMed : 32302571, PubMed : 32302572, PubMed : 34739333, PubMed : 36279435, PubMed : 36692217). Also acts as an ATP- and magnesium-dependent helicase : unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed : 9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed : 9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed : 9889278). Plays an essential role in innate immunity by promoting CGAS and RIGI activity (PubMed : 30510222, PubMed : 30804210). Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS (PubMed : 30510222). Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles (PubMed : 34779554). Enhances also RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA (PubMed : 30804210). May also act as a phosphorylation-dependent sequence-specific endoribonuclease in vitro : Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed : 11604510).
See full target information G3BP1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 10:1178439 PubMed37426420

2023

Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph P Clarke,Patricia A Thibault,Sakina Fatima,Hannah E Salapa,Subha Kalyaanamoorthy,Aravindhan Ganesan,Michael C Levin

Brain sciences 11: PubMed34679349

2021

Pro-Inflammatory Cytokines and Antibodies Induce hnRNP A1 Dysfunction in Mouse Primary Cortical Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Muxue Li,Rachel Hamilton,Hannah E Salapa,Michael C Levin

International journal of molecular sciences 22: PubMed33809384

2021

Multiple Sclerosis-Associated hnRNPA1 Mutations Alter hnRNPA1 Dynamics and Influence Stress Granule Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph-Patrick W E Clarke,Patricia A Thibault,Hannah E Salapa,David E Kim,Catherine Hutchinson,Michael C Levin

Nature communications 11:6041 PubMed33247108

2020

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Savastano,Alain Ibáñez de Opakua,Marija Rankovic,Markus Zweckstetter

Head & neck 38:1074-84 PubMed26879675

2016

Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nghia Nguyen,Emily Bellile,Daffyd Thomas,Jonathan McHugh,Laura Rozek,Shama Virani,Lisa Peterson,Thomas E Carey,Heather Walline,Jeffery Moyer,Matthew Spector,Daniel Perim,Mark Prince,Scott McLean,Carol R Bradford,Jeremy M G Taylor,Gregory T Wolf
View all publications
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Product promise

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