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AB310663

Alexa Fluor® 594 Anti-Geminin antibody [EPR23754-87]

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Rabbit Recombinant Monoclonal Geminin antibody - conjugated to Alexa Fluor® 594.

View Alternative Names

Geminin, Gmnn

  • 660 APC

    APC Anti-Geminin antibody [EPR23754-87]

  • HRP

    HRP Anti-Geminin antibody [EPR23754-87]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Geminin antibody [EPR23754-87]

  • Unconjugated

    Anti-Geminin antibody [EPR23754-87]

  • 578 PE

    PE Anti-Geminin antibody [EPR23754-87]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Geminin antibody [EPR23754-87]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Geminin antibody [EPR23754-87]

  • Carrier free

    Anti-Geminin antibody [EPR23754-87] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Geminin antibody [EPR23754-87]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Geminin antibody [EPR23754-87]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23754-87

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Geminin also referred to as GMNN is a protein that regulates DNA replication. It has approximately 25 kDa mass. Geminin inhibits DNA replication by preventing the loading of the mini-chromosome maintenance complex onto DNA during the cell cycle. This function ensures that DNA replication takes place only once per cell cycle. Geminin expresses mainly in tissues with high proliferative activity such as the developing nervous system and hematopoietic cells. Its interaction with other proteins plays a significant role in controlling cell cycle progression.
Biological function summary

In a cellular context Geminin forms part of a complex with the nuclear protein Cdt1. This complex is essential in the regulation of the cell cycle's S phase. Geminin's function restricts DNA replication by binding to Cdt1 and stopping its activity which is necessary during the G1/S phase transition. By doing so it preserves the integrity of genetic information and prevents re-replication. Geminin's inhibition remains strict until it is degraded during mitosis allowing Cdt1 to resume its function in the following cell cycle.

Pathways

The regulation of Geminin is important to maintaining proper DNA replication and cell cycle integrity. Geminin plays a role in the cell cycle control pathway particularly during the G1 to S phase transition. Additionally it interfaces with other proteins like Paxillin which is involved in cell adhesion and movement. Although not directly linked in a pathway Paxillin's role in regulating cellular dynamics can influence cell cycle processes in which Geminin is involved. Geminin also serves as a K blocker relevant in maintaining genetic stability.

Aberrations in Geminin activity can lead to severe conditions such as cancer. Its overexpression or misregulation has a link to various tumor types due to its effect on uncontrolled cell proliferation. Geminin's interaction with proteins like Paxillin and Cyclin-dependent kinases also relates to cancer progression as these interactions may involve changes in cellular adhesion and cycle checkpoints. Therefore understanding Geminin's role offers valuable insight for potential therapeutic targets in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC) (PubMed : 12192004, PubMed : 9635433). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle (PubMed : 12192004, PubMed : 9635433). Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing (By similarity). Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control (By similarity).
See full target information Gmnn
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB273633

Anti-Geminin antibody [EPR23754-87] - BSA and Azide free

primary-antibodies

geminin-antibody-epr23754-87-bsa-and-azide-free-ab273633

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