Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

26 product images

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining using rabbit Anti-GFAP antibody

  IHC image of GFAP staining in a section of frozen normal rat dentate gyrus.
  

  The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab201732 at 1/100 (pseudocolored in red) and counterstained using Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat hippocampus (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (perfused fixed) tissue labeling GABRA4 with Anti-GABRA4 antibody [EPR28365-209] ab323502 at 1/50 (10.24 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-GABRA4 (Anti-GABRA4 antibody [EPR28365-209] ab323502, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-GABRA4 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-GABRA4 antibody [EPR28365-209] ab323502 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse hippocmapus (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocmapus (fresh frozen) tissue labeling Munc18-1 with Anti-Munc18-1 antibody [EPR27956-86] ab315893 at 1/50 (9.8 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Munc18-1 (Anti-Munc18-1 antibody [EPR27956-86] ab315893, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, red) on mouse hippocampus.
  
  Panel B: anti-Munc18-1 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc18-1 antibody [EPR27956-86] ab315893 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat hippocmapus (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocmapus (fresh frozen) tissue labeling Munc18-1 with Anti-Munc18-1 antibody [EPR27956-86] ab315893 at 1/50 (9.8 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Munc18-1 (Anti-Munc18-1 antibody [EPR27956-86] ab315893, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, red) on rat hippocampus.
  
  Panel B: anti-Munc18-1 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc18-1 antibody [EPR27956-86] ab315893 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse cerebrum (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (perfused fixed) tissue labeling GDAP1 with Anti-GDAP1 antibody [EPR29581-48] ab323844 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-GDAP1 (Anti-GDAP1 antibody [EPR29581-48] ab323844, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (ab201732, magenta) on mouse cerebrum.
  
  Panel B: anti-GDAP1 stained on mouse cerebrum.
  
  Panel C: anti-NeuN stained in neurons of mouse cerebrum.
  
  Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.
  
  The section was incubated in two rounds of staining: in the order of Anti-GDAP1 antibody [EPR29581-48] ab323844 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat cerebrum (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (perfused fixed) tissue labeling GDAP1 with Anti-GDAP1 antibody [EPR29581-48] ab323844 at 1/50 (10.08 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-GDAP1 (Anti-GDAP1 antibody [EPR29581-48] ab323844, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (ab201732, magenta) on rat cerebrum.
  
  Panel B: anti-GDAP1 stained on rat cerebrum.
  
  Panel C: anti-NeuN stained in neurons of rat cerebrum.
  
  Panel D: anti-GFAP stained in astrocytes of rat cerebrum.
  
  The section was incubated in two rounds of staining: in the order of Anti-GDAP1 antibody [EPR29581-48] ab323844 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunocytochemistry/ Immunofluorescence staining of Mouse primary neural/glia cell using rabbit Anti-GFAP antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) ab319022 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab201732 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/None dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunocytochemistry/ Immunofluorescence staining of Rat primary neural/glia cell using rabbit Anti-GFAP antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) ab319022 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in rat primary glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab201732 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/None dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling Dynorphin A with Anti-Dynorphin A antibody [EPR28632-17] ab319045 at 1/50 (10.2 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Dynorphin A (Anti-Dynorphin A antibody [EPR28632-17] ab319045, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-Dynorphin A stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Dynorphin A antibody [EPR28632-17] ab319045 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat hippocampus (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling Dynorphin A with Anti-Dynorphin A antibody [EPR28632-17] ab319045 at 1/50 (10.2 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Dynorphin A (Anti-Dynorphin A antibody [EPR28632-17] ab319045, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-Dynorphin A stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Dynorphin A antibody [EPR28632-17] ab319045 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat hippocampus (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (perfused fixed) tissue labeling ALDH1L1 with Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905 at 1/100 (5.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Aldh1l1 (Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, red) on rat hippocampus.
  
  Panel B: anti-Aldh1l1 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (perfused fixed) tissue labeling ALDH1L1 with Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905 at 1/100 (5.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Aldh1l1 (Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, red) on mouse hippocampus.
  
  Panel B: anti-Aldh1l1 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat cerebellum (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (perfused fixed) tissue labeling ER81/ETV1 with Anti-ER81/ETV1 antibody [DZR-1-29] ab322139 at 1/50 (9.94 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-ETV1 (Anti-ER81/ETV1 antibody [DZR-1-29] ab322139, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on rat cerebellum.
  
  Panel B: anti-ETV1 stained on rat cerebellum.
  
  Panel C: anti-NeuN stained in neurons of rat cerebellum.
  
  Panel D: anti-GFAP stained in astrocytes of rat cerebellum.
  
  The section was incubated in two rounds of staining: in the order of Anti-ER81/ETV1 antibody [DZR-1-29] ab322139 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse cerebrum (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling ITPR2 with Anti-ITPR2 antibody [EPR28319-63] ab320725 at 1/50 (10.62 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-ITPR2 (Anti-ITPR2 antibody [EPR28319-63] ab320725, magenta), anti-Olig2 (Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] ab225099, green) and anti-GFAP (ab201732, grey) on mouse cerebrum.
  
  Panel B: anti-ITPR2 stained on mouse cerebrum.
  
  Panel C: anti-Olig2 stained in oligodendrocyte of mouse cerebrum.
  
  Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.
  
  The section was incubated in two rounds of staining: in the order of Anti-ITPR2 antibody [EPR28319-63] ab320725 and Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] ab225099, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat cerebrum (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh frozen) tissue labeling ITPR2 with Anti-ITPR2 antibody [EPR28319-63] ab320725 at 1/50 (10.62 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-ITPR2 (Anti-ITPR2 antibody [EPR28319-63] ab320725, magenta), anti-Olig2 (Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] ab225099, green) and anti-GFAP (ab201732, grey) on rat cerebrum.
  
  Panel B: anti-ITPR2 stained on rat cerebrum.
  
  Panel C: anti-Olig2 stained in oligodentrocytes of rat cerebrum.
  
  Panel D: anti-GFAP stained in astrocytes of rat cerebrum.
  
  The section was incubated in two rounds of staining: in the order of Anti-ITPR2 antibody [EPR28319-63] ab320725 and Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] ab225099, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse cerebellum (perfused-fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Mouse cerebellum (perfused-fixed) tissue labeling Ppp1r17 with Anti-Ppp1r17 antibody [EPR29124-227] ab317831 at a 1/500 (1.046 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution.

  Panel A: merged staining of anti-Ppp1r17 (Anti-Ppp1r17 antibody [EPR29124-227] ab317831, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on mouse cerebellum.
  Panel B: anti-Ppp1r17 stained on mouse cerebellum.
  Panel C: anti-NeuN stained in neurons of mouse cerebellum.
  Panel D: anti-GFAP stained in astrocytes of mouse cerebellum.

  The section was incubated in two rounds of staining: in the order of Anti-Ppp1r17 antibody [EPR29124-227] ab317831 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat cerebellum (perfused-fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Rat cerebellum (perfused-fixed) tissue labeling Ppp1r17 with Anti-Ppp1r17 antibody [EPR29124-227] ab317831 at a 1/500 (1.046 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution.

  Panel A: merged staining of anti-Ppp1r17 (Anti-Ppp1r17 antibody [EPR29124-227] ab317831, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on rat cerebellum.
  Panel B: anti-Ppp1r17 stained on rat cerebellum.
  Panel C: anti-NeuN stained in neurons of rat cerebellum.
  Panel D: anti-GFAP stained in astrocytes of rat cerebellum.

  The section was incubated in two rounds of staining: in the order of Anti-Ppp1r17 antibody [EPR29124-227] ab317831 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (perfused fixed) tissue labeling SCRN1 with Anti-SCRN1 antibody [EPR29587-538] ab323711 at 150 (9.96 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Secernin-1 (Anti-SCRN1 antibody [EPR29587-538] ab323711, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-Secernin-1stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-SCRN1 antibody [EPR29587-538] ab323711 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat hippocampus (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (perfused fixed) tissue labeling SCRN1 with Anti-SCRN1 antibody [EPR29587-538] ab323711 at 150 (9.96 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Secernin-1 (Anti-SCRN1 antibody [EPR29587-538] ab323711, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-Secernin-1 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-SCRN1 antibody [EPR29587-538] ab323711 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling Synaptojanin with Anti-Synaptojanin antibody [EPR28576-183] ab323848 at 1/50 (10.62 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Synaptojanin-1 (Anti-Synaptojanin antibody [EPR28576-183] ab323848, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-Synaptojanin-1 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Synaptojanin antibody [EPR28576-183] ab323848 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat hippocampus (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling Synaptojanin with Anti-Synaptojanin antibody [EPR28576-183] ab323848 at 1/50 (10.62 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Synaptojanin-1 (Anti-Synaptojanin antibody [EPR28576-183] ab323848, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-Synaptojanin-1 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Synaptojanin antibody [EPR28576-183] ab323848 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling GFAP with ab201732 at 1/50 (10 ug/ml) dilution. The nuclear counterstain was DAPI (Blue).

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling Neogenin with Anti-Neogenin antibody [EPR30444-522] ab324107 at 1/50 (9.76 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Neogenin (Anti-Neogenin antibody [EPR30444-522] ab324107, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-Neogenin stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Neogenin antibody [EPR30444-522] ab324107 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse cerebrum (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling Agrin with Anti-Agrin antibody [RM2082] ab324022 at 1/50 (9.98 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-AGRN (Anti-Agrin antibody [RM2082] ab324022, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (ab201732, magenta) on mouse cerebrum.
  
  Panel B: anti-AGRN stained in endothelial cells on mouse cerebrum.
  
  Panel C: anti-NeuN stained in neurons of mouse cerebrum.
  
  Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.
  
  The section was incubated in two rounds of staining: in the order of Anti-Agrin antibody [RM2082] ab324022 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (perfused fixed) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (perfused fixed) tissue labeling GABRA4 with Anti-GABRA4 antibody [EPR28365-209] ab323502 at 1/50 (10.24 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-GABRA4 (Anti-GABRA4 antibody [EPR28365-209] ab323502, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-GABRA4 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-GABRA4 antibody [EPR28365-209] ab323502 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (ab201732)

  GFAP Immunohistochemistry (Frozen sections) staining of Rat hippocampus (fresh frozen) using rabbit Anti-GFAP antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling Neogenin with Anti-Neogenin antibody [EPR30444-522] ab324107 at 1/50 (9.76 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Neogenin (Anti-Neogenin antibody [EPR30444-522] ab324107, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-Neogenin stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Neogenin antibody [EPR30444-522] ab324107 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).