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AB320437

Alexa Fluor® 594 Anti-Integrin beta 4 antibody [EPR8559]

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Rabbit Recombinant Monoclonal Integrin beta 4 antibody - conjugated to Alexa Fluor® 594.

View Alternative Names

CD104, Integrin beta-4, GP150, ITGB4

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Integrin beta 4 antibody [EPR8559]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Integrin beta 4 antibody [EPR8559]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Integrin beta 4 antibody [EPR8559]

  • 578 PE

    PE Anti-Integrin beta 4 antibody [EPR8559]

  • 660 APC

    APC Anti-Integrin beta 4 antibody [EPR8559]

  • Unconjugated

    Anti-Integrin beta 4 antibody [EPR8559]

  • Carrier free

    Anti-Integrin beta 4 antibody [EPR8559] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8559

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.
Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).
See full target information ITGB4

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com