Rabbit Recombinant Monoclonal Interferon gamma antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Interferon gamma antibody [EPR23991-53] (AB317059), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-594-interferon-gamma-antibody-epr23991-53-ab317059--flow-cytometry-intracellular-img428875.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Interferon gamma antibody [EPR23991-53] (AB317059)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
Alternative names
Interferon gamma, IFN-gamma, Immune interferon, IFNG
Recommended products
Rabbit Recombinant Monoclonal Interferon gamma antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23991-53
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Interferon gamma antibody [EPR23991-53] (ab317059), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-594-interferon-gamma-antibody-epr23991-53-ab317059--flow-cytometry-intracellular-img428875.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Interferon gamma antibody [EPR23991-53] (ab317059)")
Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Interferon gamma antibody [EPR23991-53] (ab317059)
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / Human PBMC (human peripheral blood mononuclear cell) treated with cell stimulation cocktail for 6 hours (Middle) / Untreated control (Right) cells labelling Interferon gamma with ab317059 at 1/500 dilution (0.1ug)/ Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208568) / Left isotype control.
Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG or ab317059.
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Product protocols
- Visit the general protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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