AB203404

Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

Name: Anti-Islet 1 Alexa Fluor® 594 antibody [EP4182] (ab203404) IgG | Abcam
Brand: Abcam Limited
SKU: AB203404
Rating: 4 (3 reviews)

Advanced Validation
RabMAb
Recombinant
What is this?

(3 Reviews)

(1 Publication)

Rabbit Recombinant Monoclonal Islet 1 antibody - conjugated to Alexa Fluor® 594. Neural Stem Cell marker. Suitable for ICC/IF, IHC - Wmt and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

Insulin gene enhancer protein ISL-1, Islet-1, ISL1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB203404)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB203404)

ab203404 staining Islet 1 in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203404 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
660 APC

APC Anti-Islet 1antibody [EP4182] - Neural Stem Cell Marker
578 PE

PE Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
Unconjugated

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
Carrier free

Anti-Islet 1 antibody [EP4182] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4182

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human

Applications

IHC - Wmt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/500", "IHCWmt-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store at -80°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Islet 1 also known as ISL1 is a LIM-homeodomain transcription factor with a molecular mass of approximately 38 kDa. It plays an important role in regulating gene expression during the development of the nervous system and pancreas. You can find Islet 1 predominantly in motor neurons pancreatic beta cells and cardiac muscle cells. Its expression is important during embryonic stages for cell differentiation and maturation. Researchers use "Isl 1 neuronal marker" antibodies to identify this protein's presence in tissue.

Biological function summary

Islet 1 is involved in the differentiation and survival of various cell types. As part of a transcription complex it regulates downstream target genes essential for the development of motor neurons and cardiac muscle. It also contributes significantly to the developmental pathways of insulin-producing beta cells in the pancreas. The ISL1 marker is therefore an important tool for studying cell lineage and fate in developmental biology.

Pathways

Islet 1 is involved in key developmental signaling pathways including the Notch signaling and Wnt signaling pathways. In these pathways Islet 1 collaborates with other proteins like Lhx3 and Ngn2 to ensure proper gene activation for neuronal and cardiac development. Wnt signaling affects the expression of isl 1 marker genes contributing to precise spatial and temporal regulation during embryogenesis.

Islet 1 has links to congenital heart defects and type 2 diabetes. Mutations or altered expression of this protein can disrupt pancreatic beta cell function and motor neuron development. In congenital heart disease the interaction of Islet 1 with proteins like Nkx2.5 is critical. Its role in type 2 diabetes ties with the functioning of other proteins involved in insulin secretion making it a potential target for therapeutic intervention in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).

See full target information ISL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e37227 PubMed39296007

2024

Molecular basis of CX-5461-induced DNA damage response in primary vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Liu,Guopin Pan,Jing Zhang,Jianli Wang,Xiaosun Guo,Ye Chen,Xiaoyun Wang,Xiaopei Cui,Huiqing Liu,Fan Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (AB203404)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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