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AB310421

Alexa Fluor® 594 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

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Rabbit Recombinant Monoclonal Lamin B1 antibody - conjugated to Alexa Fluor® 594.

View Alternative Names

LMN2, LMNB, LMNB1, Lamin-B1

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • 578 PE

    PE Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • 660 APC

    APC Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • HRP

    HRP Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • Unconjugated

    Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

  • Carrier free

    Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4068

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.

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Product details

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamin A Lamin B1 and Lamin C are nuclear envelope proteins critical for nuclear structure and stability. Lamin A and Lamin C both originating from the LMNA gene have molecular masses of about 74 kDa and 65 kDa respectively due to alternative splicing. Lamin B1 encoded by the LMNB1 gene has a molecular mass of around 67 kDa. These lamins are found in the nuclear lamina a dense fibrillar network lining the inner surface of the nuclear envelope. They anchor chromatin to the nuclear envelope and play roles in DNA replication repair and gene expression.
Biological function summary

Lamins play essential roles in maintaining cell integrity and regulating gene expression. Lamin A and Lamin C part of the type A lamins and Lamin B1 part of the type B lamins contribute to nuclear organization and mechanical support. These proteins form complexes within the lamina network and with other nuclear proteins participating in chromatin organization and gene transcription. Defects in lamins negatively impact cellular functions and can lead to altered gene expression patterns.

Pathways

Lamins interact with multiple signaling pathways that govern cellular homeostasis and stress response. They play roles in the mechanotransduction pathway influencing how cells perceive mechanical stress and respond to it. Interaction with proteins like emerin and nuclear actin further connects lamins to pathways involved in gene regulation and structural support of the nucleus.

Lamin dysfunction has associations with several human diseases including laminopathies such as Hutchinson-Gilford progeria syndrome and muscular dystrophy. Mutations in the LMNA gene affecting Lamin A and Lamin C often lead to disrupted nuclear architecture and impaired cellular function. Lamin B1 when overexpressed or mutated has links to neurological disorders like autosomal dominant leukodystrophy. These associations highlight the importance of lamins in disease mechanisms and provide insights into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 28716252, PubMed : 32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 28716252, PubMed : 32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 28716252, PubMed : 32910914).
See full target information LMNB1
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Product promise

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